Xiaohuang Qudan decoction alleviates ANIT-induced cholestatic liver injury by inhibiting the JAK2/STAT3 pathway and regulating TH17/Treg  

作　　者：Zhangkui Tan Lifeng Chen Zhiqin Ye Qiping Lu 

机构地区：[1]Department of Rheumatology and Immunology,General Hospital of Central Theater Command of the People’s Liberation Army,Wuhan 430070,China [2]Department of Rheumatology,Hubei Provincial Hospital of Traditional Chinese Medicine,affiliated with Hubei University of Chinese Medicine,Wuhan 430061,China [3]Department of General Surgery,General Hospital of Central Theater Command of the People’s Liberation Army,Wuhan 430070,China

出　　处：《Chinese Journal of Natural Medicines》2025年第4期457-470,共14页中国天然药物(英文版)

基　　金：supported by the Natural Science Foundation of Hubei Province(No.2022CFD020);the Postdoctoral Research Initiation Fund(No.20211015KY19);the Hubei Provincial Medical Young Top Talents[No.2019(48)]。

摘　　要：Xiaohuang Qudan decoction(XHQDD)is a classical traditional Chinese medicine(TCM)formula widely used in the treatment of cholestatic liver injury.Despite its widespread use,the protective mechanism of XHQDD against cholestatic liver injury remains incompletely understood.The aim of this study was to investigate whether XHQDD mediates its beneficial effects by inhibiting the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)pathway and regulating TH17/Treg balance.To this end,the researchers used Sprague-Dawley(SD)rats and established a cholestatic liver injury model by oral administration of alpha-naphthylisothiocyanate(ANIT).The experimental group was divided into six groups:Control(CON),ANIT,ursodeoxycholic acid(UDCA),XHQDD-low dose(XHQDD-L)group,XHQDD-medium dose(XHQDD-M)group,and XHQDD-high dose(XHQDD-H)groups.Then,after 7 d of treatment,various tests were performed to verify the results.Firstly,XHQDD and its drug-containing serum were analyzed by ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry(UPLC-MS/MS),and 14 blood-entry components were identified.Then,bile flow was monitored and found to be significantly reduced in the model group,which was significantly reversed in the UDCA and XHQDD groups.To further assess ANIT-induced liver injury,hematoxylin and eosin(H&E)and Sirius red staining,alongside transmission electron microscopy(TEM),were employed to observe liver tissues,revealing hepatocellular injury,cholestasis,and hepatic fibrotic changes.Serum inflammatory factors and liver injury indicators were assessed using enzyme-linked immunosorbent assay(ELISA),indicating an inflammatory state in ANIT-induced liver injury rats.The expression levels of JAK2/STAT3-related genes and proteins in liver and intestinal tissues were measured via quantitative reverse transcription polymerase chain reaction(qRT-PCR),immunohistochemistry,immunofluorescence(IF)staining,and Western blotting(WB)assays.These studies revealed that the inflammatory state of liver-injur

关 键 词：ANIT TH17/TREG Cholestatic liver injury Xiaohuang Qudan decoction JAK2/STAT3 UHPLC-MS/MS 

分 类 号：R285.5[医药卫生—中药学]