糖尿病肾病大鼠肝DNA甲基化酶活性及基因组DNA对DNase敏感性的分析  

ACTIVITY OF DNA METHYLASE AND DNASE SENSITIVITY ASSAY OF GENOME DNA OF LIVER FROM RAT OF DIABETIC NEPHROPATHY

作　　者：汪琛颖[1] 马晴[1] 何忠效[1] 

机构地区：[1]北京师范大学生命科学学院

出　　处：《北京师范大学学报（自然科学版）》2002年第6期810-814,共5页Journal of Beijing Normal University(Natural Science)

基　　金："九五"国家科技攻关资助项目 (96 - 90 6 - 0 9- 0 7)

摘　　要：用 3H标记的 S-腺苷酰 - L -甲硫氨酸 (3H- SAM)作为甲基供体 ,以同位素掺入法测定了正常大鼠、糖尿病肾病大鼠、中药“糖微康”治疗的糖尿病肾病大鼠、西药“开博通”治疗的糖尿病肾病大鼠的肝细胞的 DNA甲基化酶活性 ,并对从以上各组动物肝组织中提取的基因组 DNA分别进行 DNase 敏感性分析 .结果显示 :糖尿病肾病大鼠肝 DNA甲基化酶活性明显低于正常鼠肝 ,其基因组 DNA对 DNase 敏感性比正常大鼠肝增强 ;中药“糖微康”治疗及西药“开博通”治疗的大鼠的肝 DNA甲基化酶活性均有所回升 ,且中药组更接近正常大鼠对 DNase 的敏感性 .The activity of DNA metlylase is measured from control animals,animals of Diabetic Nephropathy, animals of Diabetic Nephropathy treated with Chinese traditional medicine 'Tang Wei Kang', and animals of Diabetic Nephropathy treated with medicine Capton by measuring incorperation of 3H-methyl. 3H-S-adenosyl-L-methionine ( 3H-SAM) is used as methyl donor. At the same time, the sensitivity of DNA from different rat liver to DNase Ⅰ is mersured. The results demonstrate that DNA methylase activity of rat liver of Diabetic Nephropathy is obviously lower than the control, and its genome DNA possesses higher sensitivity to Dnase I than that of the control. However, DNA methylase activity of rat liver of Diabetic Nephropathy treated with Chinese traditional medicine 'Tang Wei Kang' or the medicine Capton is all reversed to the control level. Meanwhile, the sensitivity of the former's DNA to DNase I is closely to the control.

关 键 词：大鼠 肝 酶活性 基因组DNA 敏感性 糖尿病肾病 DNA甲基化酶 DNaseⅠ 糖微康 中药 药理机制 

分 类 号：R587.2[医药卫生—内分泌] R285.[医药卫生—内科学]