水稻成熟胚盾片诱导愈伤组织再生体系的建立  

Establishment of A Rice Transformation System by Regeneration of Embryonic Callus Induced from Mature Embryo Scutum

作　　者：伍成祥[1,2] 宛煜嵩[3] 徐俊[1] 苏金[4] 方宣钧[1,2] 

机构地区：[1]海南省热带农业资源开发利用研究所 [2]中国农业科学院生物技术研究所北京100081 [3]海南省农作物分子育种重点实验室 [4]中国农业科学院生物技术研究所

出　　处：《热带作物学报》2002年第3期88-95,共8页Chinese Journal of Tropical Crops

基　　金：国家863计划(批准号:2001AA211091);海南省自然科学基金项目(批准号:3003);2001年海南省科学事业费项目资助项目

摘　　要：采用水稻成熟胚盾片诱导愈伤组织作为分化再生的外植体,通过优化激素组合和调节培养基渗透压,建立了适合水稻遗传转化的高效再生体系.将水稻成熟胚诱导愈伤组织分化再生的过程划分为3个不同时期,即成熟胚盾片愈伤组织诱导,胚性细胞诱导保持,胚性愈伤组织分化再生.以Culture I(LS+2,4-D 2.0 mg/L)诱导成熟胚盾片愈伤组织,愈伤组织剥离后接种于CultureⅡ-3(LS+2,4-D 2.5 mg/L+山梨醇3g/L)上诱导胚性愈伤组织,继代后接种于CultureⅢ-2(MS+NAA 0.5 mg/L+6-BA1.0 mg/t+Kinetin 2.0 mg/L+山梨醇8g/L)上分化成苗.培养结果表明,该再生体系所获得的胚性愈伤组织分化再生率均在60%～80%之间.利用本研究建立的高效再生体系建立水稻外源基因遗传转化系统,使转化过程中所得转化愈伤组织高效再生成苗,从而顺利获得转化植株,可以提高水稻外源基因转化的效率,为水稻品种的定向遗传改良提供优良的遗传转化技术体系.Using embryonic callus induced from rice mature embryo scutum as explants for regenera tion,a highly efficient rice regeneration system suitable for rice genetic transformation was estab lished by optimizing the combinations of hormones and adjusting the osmotic pressures of the culture media.Culture I(LS+2,4-D2.0mg /L)was used to induce callus from mature embryo,then the callus was cultivated on Culture II-3(LS+2,4-D2.5mg /L+Sorbito l3g/L),and finally plants were regenerated on Culture III-2(MS +NAA0.5mg /L+6-BA1.0mg /L+Kinetin2.0mg /L+Sorbitol8g/L).In the cultivation of embryonic callus from5varieties of rice by this regeneration system,the regeneration rate of each variety ranged from60to80percent.The highly efficient regenera tion system can be used to set up the rice genetic transformation system.The transformed callus gained in the transformation process can regenerate plants efficiently,and the efficiency of rice genetic transformation can be enhanced,thus providing a superb genetic transformation technological system for the oriented genetic improvement of rice varieties.

关 键 词：水稻 成熟胚 盾片诱导 再生体系 愈伤组织 

分 类 号：S511[农业科学—作物学]