豚鼠耳蜗局灶性微循环障碍模型的建立  

Restricted microcirculation disorder induced by photochemical reaction in the guinea pig cochlea

作　　者：钟贞[1] 李学佩[2] 吴红涛[3] 吴长松 

机构地区：[1]北京大学第一医院耳鼻咽喉头颈外科,北京100034 [2]北京大学第三医院耳鼻咽喉头颈外科 [3]九江市第一人民医院耳鼻咽喉科 [4]吉化集团公司总医院一院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2003年第1期40-43,共4页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :探讨耳蜗局灶性微循环障碍对耳蜗血流、听功能及耳蜗形态学的影响。方法 :采用光化学诱导法 ,经豚鼠颈外静脉注射 2 %四碘四氯荧光素二钠 (RB)后 ,用 (5 4 0± 4 0 )nm的绿色光照射耳蜗第 2转外侧壁(范围约 1.2mm× 1.0mm) ,诱导该部位血管纹微血栓形成。结果 :诱导后观察 5h ,被照射部位的耳蜗血流随时间逐渐下降 ,动作电位阈移值逐渐提高 ,二者的变化呈负相关。耳蜗铺片显示 ,被照射部位诱导后 30min耳蜗外侧壁毛细血管扩张 ,部分毛细血管轮廓不清、血流中断 ;诱导后 90～ 30 0min ,大片的毛细血管萎缩、闭塞、数目减少 ;诱导 3h后 ,光照区内部分外毛细胞、内毛细胞坏死缺失 ,外毛细胞病变较内毛细胞为重 ,毛细胞坏死区的长度为 (115 2 .5 0± 36 3.2 6 ) μm(n =4 )。非光照区CBF及形态学均无明显变化。结论 :光化学法可诱导耳蜗外侧壁局限性微血管损伤 ,造成豚鼠听力下降 ,为耳蜗不同部位微循环障碍导致不同类型听力损伤的研究提供了实验依据 ,并为耳蜗血栓性疾病探索新的治疗方法提供了动物模型。Objective:To investigate the effect of restricted cochlear microcirculation disorder on the cochlear blood flow, auditory function, and morphology.Method:Photochemical reaction was utilized to induce localized microcirculation damage to the second cochlear turn. After systemical administration of rose bengal, the second cochlear turn was illuminated with green light (wave length 540 nm±40 nm) to cause thrombosis in the stria vascularis, the area was about 1.2 × 1.0 mm 2.Result:At the site of the illumination, cochlear blood flow gradually decreased, and the threshold of action potential gradually increased. Surface preparing showed thrombus was in the stria vascularis. Three hours after the photochemical reaction, some hair cells in the illuminated area necrotized. The length of the lesion was 1152.50 ± 363.26 μm (n=4). But hair cells in the non illuminated area were normal.Conclusion:The photochemical method is a reliable tool to produce an animal model with the restricted microvessel lesion in the lateral wall of the cochlea. It could be used as an animal model for the treatment of the cochlear thrombosis.

关 键 词：豚鼠 耳蜗 微循环 疾病模型 动物 

分 类 号：R322.92[医药卫生—人体解剖和组织胚胎学]