PKC-α和PKC-δ在不同发育阶段小鼠睾丸中的差异性表达  

Differential Expression of PKC-α and PKC-δ in Testes of Mouse at Various Developmental Stages

作　　者：冯晨[1] 付伟[1] 赵咏梅[1] 陈菲[1] 宗志宏[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学教研室,沈阳110001

出　　处：《中国生物化学与分子生物学报》2002年第6期780-782,共3页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重点基础研究发展规划 (973 )项目 (G 19990 5 5 90 0 2 )资助~~

摘　　要：Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.

关 键 词：PKC-Α PKC-δ 发育阶段 小鼠睾丸 差异性表达 

分 类 号：Q954.434[生物学—动物学] Q49