低钙饮食对大鼠胫骨组织形态的影响  

The Effect of Low Calcium Diet on Histomorphology of Tibia in Rats

作　　者：莫梅英[1] 陈建波[1] 黄莲芳[1] 冷扬[1] 李青南[1] 

机构地区：[1]广东医学院实验动物中心,湛江524023

出　　处：《上海实验动物科学》2002年第4期227-230,共4页Shanghai Laboratory Animal Science

摘　　要：为观察低钙饮食对雌雄大鼠所致骨骼的变化,将36只SD大鼠(雌雄各半),按性别各随机分成正常年龄对照组(control)、极低钙组(VLCD)(Ca 0.01％)和低钙组LCD(Ca0.3％)。三月后取左侧胫骨近心端,行骨组织形态计量学分析。结果为①与对照组相比,VLCD和LCD雄性大鼠骨量减少,骨小粱面积百分率(％Tb.Ar)分别减少57％(P<0.01％)和40％(P<0.05％),骨小粱数量(Tb.N)、骨小粱分离度(Tb.Sp)均显著增加,骨结构变差。而雌性大鼠仅VLCD组大鼠骨量减少,％Tb.Ar减少36％(P<0.01％),骨结构同样变差,其程度较雄性大鼠有所减轻;②在VLCD组,雌雄大鼠其骨形成指标荧光周长百分数(L.Pm％)、矿化沉积率(MAR)、骨形成率(BFR/BV)均有增加的趋势,而骨吸收参数骨小粱周长破骨细胞数量(N.OC/Tb.Pm)在雌雄大鼠分别增加56％和66％(P<0.05％),呈现骨高转化的改变。LCD组骨形成变化在雌雄大鼠均不明显,骨吸收增加,但没有显著意义。结论:低钙饮食使大鼠骨量减少并伴随着骨转换的增加,其影响的程度随缺钙的程度增加而增加。雄性大鼠对低钙饮食更为敏感。To compare the effects of low calcium diet on bone in rats by bone histomor-phometry. Thirty-six 3-month-old Sprague-Dawley male and female rats (each half) were randomly divided into three groups, control group, very low calcium diet group (VLCD) and low calcium diet group (LCD). After 90 days, all rats were killed. The proximal tibia (PTM) was harvested for histomorphometric analysis. The results revealed that the VLCD group male and female rats had lost 57%(P<0. 01) and 40%(P <0. 05) of cancellous bone compared with that of control group by 3 months respectively. Dynamic data in male and female rats showed that bone formation indices such as percent labeled perimeter(L. Pm%),mineral apposition rate (MAR) and bone formation rate(BFR/BV) all increased without significance. Bone resorption indices such as osteo-clast number per mm (Oc. N/Tb. Pm) increased 63%(P<0.01)and 56%(P<0. 01). The effect on LCD was lessen than in VLCD and there is significant bone loss only in male rats. The results indicated that bone loss was associated with increased bone turnover in low calcium dietary rats. The male rats were more sensitive to low calcium diet than female rats.

关 键 词：低钙饮食 大鼠 胫骨组织形态 影响 骨质疏松 

分 类 号：R58[医药卫生—内分泌]