凝血因子IX基因剔除小鼠的遗传及血液学指标检测  

Genetic Analysis and Blood Assay of Coagulation Factor -IX Knockout Mouse Strain

作　　者：郝光荣[1] 孙伟[1] 陈立[2] 崔淑芳[1] 汤球[1] 黄立志[1] 曹平[1] 

机构地区：[1]第二军医大学实验动物中心,上海200433 [2]复旦大学遗传所

出　　处：《中国实验动物学报》2002年第4期233-235,共3页Acta Laboratorium Animalis Scientia Sinica

基　　金：上海市科学技术发展基金资助项目 9949190 3 2

摘　　要：目的　鉴定凝血因子IX基因剔除小鼠。方法　采用PCR扩增检测小鼠的DNA样品以及采用一期法检定小鼠血浆FIX活性和血浆凝血酶原时间 (plasmaprothrombintime ,PT)、白陶土部分凝血活酶时间 (Kaolinpartialthromboplastintime ,KPTT)值。结果　小鼠PCR检测为阳性 ,FIX活性 <5 %。结论　凝血因子IX基因剔除小鼠能稳定遗传 ,鉴定结果提示该小鼠符合人血友病B相应临床症状。ObjectiveTo assay the coagulation factor IX -knockout mice. MethodsThe DNA of mice was analysed by PCR and the plasma of the mice was measured by one stage method. ResultsPCR analysis of FIX mice was positive and FIX activity was below 5%. ConclusionThe result showed that the coagulation FIX -knockout mice could be generated steadily and exhibited a phenotype similar to human hemophilia B.

关 键 词：白友病B 疾病模型 凝血因子Ⅸ 基因剔除小鼠 遗传 血液学指标 检测 

分 类 号：R554.1[医药卫生—血液循环系统疾病] R332[医药卫生—内科学]