梅岭霉素高产菌株链霉素抗性基因突变株筛选  

ACQUISITION OF STREPTOMYCIN-RESISTANT( STR )MUTANTS SCREENS HIGH-YIELD MEILINGMYCIN STRAIN IN STREPTOMYCES NANCHANGENSIS

作　　者：涂国全[1] 刘姝[1] 黎循航[1] 

机构地区：[1]江西农业大学生物工程系,南昌330045

出　　处：《微生物学通报》2002年第6期33-37,共5页Microbiology China

摘　　要：通过链霉素对梅岭霉素 (Meilingmycin)产生菌南昌链霉菌NS 41 80菌株孢子致死浓度的测定 ,采用诱变剂EMS 4种不同剂量对菌株孢子进行诱变处理 ,然后涂布在含链霉素致死浓度的高氏平板上 ,获得了大量的链霉素抗性基因 (str)突变株。并进一步筛选到梅岭霉素高产菌株 80 5 1 1 2 2 1 ,在摇瓶条件下 ,只产梅岭霉素不产南昌霉素 ,梅岭霉素活性单位达 1 ,52 1 μg/mL,比NS 41 80的摇瓶发酵单位 855μg/mL提高了 77 9% ,该菌株连续传 6代进行摇瓶发酵 ,其F2 代和F3 代发酵单位稳定 ,F4代至F6 代随着代数增加 ,梅岭霉素发酵单位急速下降。通过EMS诱变剂量分别与抗药性突变率和str突变株产梅岭霉素产量的产势统计分析表明 ,菌株抗药性突变与产量突变密切相关。产量突变的EMS剂量高于str突变剂量 ,从而建立了梅岭霉素产生菌链霉素抗性基因突变筛选方法。After testing the resistance of Streptomycin to the strain NS-41-80 which is the Meilingmycin producer- Streptomyces nanchangensis , a lot of streptomycin-resistant ( str ) mutants were screened after the spores regenerated on the lethal media when they were treated with 4 different dosage of super-mutagent EMS. We have abtained the high-yield strain 80-5. 11-221 from these str mutants which only produces Meilingmycin and no Nanchangmycin in the rotation-flask experiments. The productivity of 80-5. 11-221 is 1521μg/mL 77. 9% higher than CK's 855μg/mL. After the shake flask fermentation experiment of 80-5. 11-221 for six generations, the productivity of F 2 and F 3 is stable and the productivity of F 4?F 5?F 6 decreases hastily with the increment of generations. It was showed that the chemical-resistance mutation of the strains had closely relation with yield mutation and the EMS dosage of the yield mutation was higher than the resistance-chemical mutation's through the statistical analysis between the dosage of mutation and the chemical-resistance mutational frequency?the variate capacity of the mutants' yield. A model of the resistance-chemical mutational labelling rational selection of the strain producing Meilingmycin was established.

关 键 词：南昌链霉菌 杀虫抗生素 梅岭霉素 高产菌株 链霉素抗性基因突变株 筛选 

分 类 号：Q939.9[生物学—微生物学]