扶正祛邪中药复方对猴艾滋病模型实验研究  

Study on Effect of Fuzheng Quxie Chinese Drugs on Monkey of SIV Infection Moden

作　　者：徐淑玲[1] 关崇芬[1] 翁新愚[1] 吴小娴[2] 卢耀增[2] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700 [2]中国医学科学院实验动物研究所,北京100021

出　　处：《中国实验方剂学杂志》2003年第1期42-44,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：扶正祛邪中药复方“艾克冲剂”对猴SIV感染模型 (SAIDS)治疗作用。方法 :利用SIVmac病毒液静脉接种恒河猴造成猴艾滋病实验模型 ,流式细胞仪测定感染及治疗动物外周血CD+4细胞、CD+8细胞数及CD+4 CD+8比值 ,并以MTT法观察T、B细胞对有丝分裂原诱导分化增殖反应。结果 :艾克冲剂能提高CD+4细胞百分数及CD+4 CD+8比值 ,并能促进T、B淋巴细胞增殖。结论 :扶正祛邪中药艾克冲剂能够提高、改善SIVmac感染动物免疫功能。Objective:To Investigate the effect of Lyke Granule on simian immunodeficiency Virus(SIV) infection model. SAIDS model were used. Using monoclonal antibody label on CD + 4 and CD + 8 cells in PBMC with direct immunofluorescence,the percentage was analyzed by flow cytometry and analyzed CD + 4/CD + 8 ratio. T and B cells proliferation induced by mitogens was defected before treatment and after treatment with Lyke Granule. Results indicate that Lyke Granule enhanced the proliferation of T and B cells induced by motogen,CD + 4 T cells were increased in PBMC and CD + 4/CD + 8 were changed.

关 键 词：扶正祛邪中药复方 艾克冲剂 SIV模型 CD4^+细胞 细胞增殖 动物模型 

分 类 号：R259.129.1[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]