不同预处理方案对异基因骨髓移植物抗宿主病的影响  

Influence of different pre-treatments over graft-versus-host disease after al logeneic bone marrow transplantation

作　　者：邓晓辉[1] 翁霞[1] 梁辉[1] 王昀[1] 陈琳军[1] 

机构地区：[1]上海第二医科大学附属新华医院血液科,上海200092

出　　处：《免疫学杂志》2003年第1期33-35,共3页Immunological Journal

基　　金：上海血液学研究所胡应洲科研基金资助项目

摘　　要：目的　建立大鼠异基因骨髓移植模型 ,应用不同的预处理方案 ,研究它们对移植物抗宿主病 (GVHD)严重程度的影响。方法　Wistar大鼠为供鼠 ,SD大鼠为受鼠 ,受鼠分 4组 ,分别予以不同的预处理方案 ,A、B组受鼠自移植前 4～ 1d ,腹腔注射氟达拉宾 (Fludarabine ,Flu) 1mg kg ,移植当天分别接受 2、6Gy的全身照射 ;C、D组受鼠在移植当天分别接受 2、6Gy的全身照射。制备供鼠骨髓细胞。照光 4h后 ,经尾静脉输注供鼠骨髓细胞 3.6× 10 7。观察各受鼠GVHD反应。结果　GVHD程度依次为 :Flu +2Gy组 <2Gy组 <Flu +6Gy组 <6Gy组。结论　采用非清髓性预处理方案及应用氟达拉宾 ,可明显缓解GVHD反应 。ObjectiveWe established a rat allogeneic bone marrow t ra nsplantation model,and investigated different effects of different regimes on t he severity of graft versus host disease after allogeneic bone marrow transpla ntation. MethodsSD rats were transplanted with Wistar rats in allo BMT model. Recipient rats were divided into 4 groups, group A and group B 1 mg/kg of fludarabine were administered intraperitoneally on days 4, 3, 2, 1 prior to transplantation. These animals received TBI 2,6 Gy 4 hours prior to BMT; Group C,D were exposed to a dose of 2,6 Gy total body irradiation respe ctively on the same time. All these recipient rats were given donor bone marrow cells ( 3.8 ×10 7) via tail vein. Afterwards we observed GVHD of each groups . Results The severity of GVHD was as follow: Flu+2 Gy group <2 Gy group <Flu+6 Gy group <6 Gy group. Conclusion It was f ound that by using non myeloablative bone marrow transplantation or fludarabine as pre treatments,the degree and severity of GVHD could be reduced and made t ransplantation more safe.

关 键 词：骨髓移植 异基因 移植物抗宿主病 预处理 动物实验 

分 类 号：R392[医药卫生—免疫学]