猪链球菌2型溶菌酶释放蛋白的黏附作用  

Role as adhesin of muramidase released protein of Streptococcus suis type 2

作　　者：曾巧英[1] 陆承平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室

出　　处：《南京农业大学学报》2002年第4期67-71,共5页Journal of Nanjing Agricultural University

基　　金：国家 973项目子课题 (G19990 1190 6)

摘　　要：为研究猪链球菌 2型 (SS2 )的毒力因子溶菌酶释放蛋白 (MRP)的黏附作用 ,进行了如下试验 :1 用黏附计数法比较菌株HA980 1(MRP+ )和SH0 0 6 4 4 4 (MRP-)的黏附动力学 ,两菌株均能黏附于HEp 2细胞 ,MRP+ 株的最大黏附菌数显著高于MRP+ 株 (P <0 0 5 ) 。 2 菌体置 5 6℃ 1h ,或用胰酶或半乳糖预处理 ,细菌黏附力完全丧失 ;分别用高碘酸、溶菌酶、秋水仙素和DNaseⅠ处理HEp 2细胞 ,前者可完全阻断HA980 1对它的黏附 ,后三者使黏附菌数显著下降。3 用纯化的MRP处理HEp 2细胞 ,或用抗MRP兔血清或MRP的单克隆抗体处理菌体 ,MRP+ 株的黏附菌数显著降低(P <0 0 5 ) ,但不能被完全抑制 ;用抗MRP-株全菌兔血清处理菌体 ,也能降低MRP+ 株的黏附菌数。 4 半乳糖或 5 6℃预处理MRP ,使其完全丧失对HA980 1的黏附抑制功能 ,但胰酶、过氧化氢和巯基乙醇的预处理没有明显的影响。结论 :MRP是SS2的一种黏附素 。The adhesion of muramidase released protein(MRP),a virulence factor of Streptococcus suis type 2(SS2)were tested.The adhesive kinetics of SS2 isolate HA9801(MRP +)and SH006444(MRP -)were compared through adhesive counting.Both strains could adhere to HEp 2 cells,but the adhesive levels of MRP + strain were significantly higher than that of MRP - strain(P<0 05).The adhesive activities of HA9801 were abolished by pretreatment of bacterium cell with trypsin or galactose,or at 56 ℃ 1 h,and by pretreatment of HEp 2 cells with periodic acid.After pretreatment of HEp 2 cells with colchicine,lysozyme or DNase Ⅰ,the adhesive levels could be reduced dramatically.The adhesive activities of MRP + strain were obviously reduced but not blocked by pretreatment of bacterum cells with antiserum or monoclonal antibodies against MRP,or against MRP - strain,as well as by pretreatment of HEp 2 cells with purified MRP.MRP was destroyed completely its inhibitive performance against adhesion of HA9801 to HEp 2 cells after pretreatment at 56 ℃ or with galactose,other than with trypsin,mercaptoethanol and hydrogen peroxide.Conclusion:MRP is an adhesin of SS2 and galactose can block its adhesion.

关 键 词：猪链球菌2型 溶菌酶 释放蛋白 黏附作用 

分 类 号：S852.611[农业科学—基础兽医学] S85[农业科学—兽医学]