水稻OsWrky基因的细胞核定位研究  

STUDY OF SUBCELLULAR LOCALIZATION OF RICE OSWRKY GENE

作　　者：齐高燕[1] 郭泽建[1] 

机构地区：[1]浙江大学生物技术研究所,杭州310029

出　　处：《山东农业大学学报（自然科学版）》2002年第4期506-508,共3页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家自然科学基金 (编号 :30 0 70 4 93)

摘　　要：绿色荧光蛋白 (GFP)基因是一种被广泛应用的报告基因。为了了解克隆的水稻WRKY(OsWrky)基因的核定位性质 ,将OsWRKY与GFP基因融合 ,并构建在Ubiquitin启动子控制的pCambia130 1载体上 (pCU -OsWrkyGFP)。利用基因枪介导的方法将pCU -OsWrkyGFP导入洋葱内表皮后 ,荧光显微镜观察GFP的发光部位仅在细胞核内 ,而作为对照的没有融合OsWRKY基因的载体 (pCU -GFP)轰击后细胞质和细胞核中都有荧光。Green fluorescent protein (GFP) is a new reporter gene and is widely used in plant research. A rice OsWrky gene was fused with GFP gene, and constructed into pCambia1301 under the control of an promoter ubiquitin. The plasmid (pCU-OsWrkyGFP) was transformed into onion epidermal cells by particle bombardment.Bright green fluorescence was visualized in the nuclear only with bombardment of pCU-OsWrkyGFP by fluorescence microscopy, whereas the ubiquitin controled GFP plasmid showed the whole cell distribution of GFP. The result suggested that OsWrky gene might function as a transcription factor in the nuclear.

关 键 词：水稻 OsWrky基因 细胞核定位 绿色荧光蛋白 转录因子 

分 类 号：S511.03[农业科学—作物学]