鸡新城疫病毒F48E9株血凝素神经氨酸酶基因免疫诱导免疫保护的初步研究  

Protection of Chickens from Newcastle Disease by Vaccination with Gene Vaccine Expressing the HN Protein of Newcastle Disease Virus

作　　者：刘胜旺[1] 孔宪刚[1] 童光志[1] 马云燕[1] 卢景良[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,国家兽医生物技术重点实验室,哈尔滨150001

出　　处：《中国农业科学》2002年第12期1565-1569,共5页Scientia Agricultura Sinica

基　　金：国家重点科技攻关计划资助项目 (96 C0 1 0 4 0 1)

摘　　要：将我国新城疫病毒标准强毒F4 8E9株的血凝素 神经氨酸酶基因克隆于真核表达载体 pcDNA3 中 ,进行鉴定后筛选阳性质粒。将此阳性质粒进行大量法提取并纯化后作为基因疫苗以每只鸡 10 0 μg的剂量肌肉接种 4 5日龄SPF鸡 ,并以 pcDNA3 空质粒作为对照。结果表明 ,新城疫基因疫苗能够诱导机体产生新城疫病毒特异的血清IgG抗体反应 ,但产生抗体所需的时间较长 ,抗体滴度的增加较为缓慢。人工接种新城疫强毒后 ,基因疫苗免疫组 4 / 6的鸡得到保护 ,而且保护鸡具有明显的抗体反应 ,2只鸡未得到保护 ,但其死亡时间明显迟于对照组鸡 ,死亡鸡具有典型的新城疫病毒感染鸡的病理形态学变化。To evaluate the effect of a DNA vaccine for chickens, we constructed a plasmid vector as DNA vaccine that expressing the Newcastle disease virus (NDV) F48E9 strain Hemagglutinin neuraminidase gene. Six chickens were injected intramusculary with 100 μg DNA vaccine . Four of the six chickens protected from lethal NDV challenge and had obvious antibody response. Two of the six chickens that immunized with DNA vaccine were not protected but their dead time were delayed. All of the chickens that had not been protected from lethal challenge had obvious pathological lesions which caused by NDV.

关 键 词：鸡新城疫病毒F48E9株 血凝素-神经氨酸酶基因 诱导 免疫保护 基因免疫 基因疫苗 

分 类 号：S852.4[农业科学—基础兽医学]