以羊膜为载体培养游离软骨细胞修复兔关节软骨缺损  

Transplantation of Isolated and Cultured Chondrocyte with Amnion as a Carrier for Repairing Articular Cartilage Defect in Rabbit

作　　者：王能兴[1] 朱争艳[1] 姚爱琳[1] 张雪华[1] 于至悌[1] 徐炜[1] 商振德[1] 卢晓栋[1] 李继钢[1] 刘智[1] 

机构地区：[1]天津医科大学附属第三中心医院骨科,天津300170

出　　处：《生物医学工程与临床》2002年第4期185-187,共3页Biomedical Engineering and Clinical Medicine

基　　金：天津市科委青年基金资助项目

摘　　要：目的　探讨膜结构为载体培养游离软骨细胞修复软骨缺损的可行性。方法　以兔羊膜为载体将体外培养的同种异体游离软骨细胞植于兔左侧股骨外踝软骨缺损区 ,分别于 4、8、12周处死动物 ,整个膝关节被解剖 ,进行大体观察、组织学评价、电镜观察及SRY基因性别鉴定 ,并以兔体的右膝关节做为对照。结果　术后 4、8、12周大体、组织学、电镜观察显示软骨缺损区新生了透明软骨 ,SRY基因性别鉴定证明新生的软骨来源于移植的同种异体软骨细胞 ;而对照组则仅见纤维组织样的修复组织。Objective The feasibility of transplantation of isolated and cultured chondrocyte with membrane structure as a carrier for repairing articular cartilage defect was studied. Methods Thirty adult rabbits were enrolled in this study. The isolated and cultured rabbit chondrocyte with rabbit amnion as carrier were transplanted homoplastically to the cartilage defect rejoin on left femur exocondyle in all the experimental rabbits. Their right knee joints were adopted as controls. Of 30 rabits 10 were sacrificed at the end of 4,8,12 weeks respectively. The gross anatomic, histologic evaluation and sex-determing region Y(SRY) sex appraisal of the grafts were performed. Results The articular cartilage defects were repaired with new hyaline cartilage from transplanted chondrocyte. The fibroid tissue was only found in the controls. Conclusion The articular cartilage defects can be repaired by transplantation of homologuous chondrocytes with amnion as a carrier.

关 键 词：羊膜 载体 体外细胞培养 游离软骨细胞 修复 兔 关节软骨缺损 

分 类 号：R687.3[医药卫生—骨科学]