细胞壁缺陷型淋病奈瑟菌cppB基因的检测  

DETERMINATION OF CRYPTIC PLASMID B GENE IN CELL WALL DEFICIENCY OF NEISSERIA GONORRHOEAE

作　　者：魏荣旋[1] 王和[1] 

机构地区：[1]贵阳医学院微生物学教研室,贵州贵阳550004

出　　处：《中国微生态学杂志》2002年第6期345-346,共2页Chinese Journal of Microecology

摘　　要：目的 :了解细胞壁缺陷对淋病奈瑟菌隐蔽性质粒B基因 (cppB)的影响。方法 :用青霉素诱导淋病奈瑟菌成L型 ,以非高渗液体培养基传代培养并获得L型纯培养物 ,用cppB基因特异性引物以聚合酶链反应 (PCR)检测不同代次稳定L型纯培养物的cppB基因。结果 :淋病奈瑟菌细菌型及其传 1～ 4代的L型培养物具有cppB基因 ,传 5代后的L型培养物不能检出cppB基因。结论 :细胞壁缺陷可造成淋病奈瑟菌隐蔽性质粒丢失 ,导致cppB基因PCR检测漏诊。Objective:To understand the influence of cell wall deficiency on the cryptic plasmid B(cppB) gene of Neisseria gonorrhoeae Methods:The N.gonorrhoeae was induced into L form by Penicillin and subcultured in non high osmotic liquid medium to get pure cultures of the L form.The cppB of the bacteria form and the L form with different generation of N.gonorrhoeae were detected by polymerase chain reaction with the specific primer of cppB gene.Results:The cppB gene could be found in the cultures of the bacteria form and the L form which were subcultured for 1～4 times,but could not be found in the cultures which were subcultured more than 5 times.Conclusion:The cppB gene of N.gonorrhoeae would be lost after the cell wall deficiency and it would make the negative diagnosis for the determination of cppB.

关 键 词：细胞壁缺陷型淋病奈瑟菌 cppB基因 检测 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学] R378.[医药卫生—临床医学]