人源抗HIV-1 gp120趋化蛋白受体结合位点Fab单克隆抗体基因的获得  

Cloning of human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor from phage Fab antibody library

作　　者：沈宏辉[1] 貌盼勇[1] 洪世雯[1] 侯俊[1] 杨健洋[1] 

机构地区：[1]解放军第三○二医院病毒研究室,北京100039

出　　处：《中华实验和临床病毒学杂志》2002年第4期357-360,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　筛选人源抗HIV 1gp12 0趋化蛋白受体结合位点噬菌体Fab单克隆抗体基因。方法　根据HIV 1gp12 0趋化蛋白受体结合位点的氨基酸序列合成 2 3肽 ,并以此为固相抗原从HIV 1噬菌体Fab抗体库筛选阳性克隆 ,并进行鉴定及序列测定。结果　获得了 1株抗HIV 1gp12 0趋化蛋白受体结合位点的人源Fab抗体克隆 ,具有较高的亲和力、特异性和抑制率 ,序列测定及分析显示该抗体属IgGⅠ亚类 ,κ型 ,重、轻链可变区分别属VhⅢ和VκⅢ基因家族。结论　成功地获得了人源抗HIV 1gp12 0趋化蛋白受体结合位点噬菌体Fab单克隆抗体基因。Objective To screen human monoclonal Fab fragments against HIV 1 gp120 peptide binding chemokine receptor. Methods A synthesized polypeptide containing 23 amino acid residues of the gp120 antigen epitope binding chemokine receptor was coated as the solid phase antigen. After biopanning from the HIV 1 phage Fab antibody library, the acquired positive clones were tested and sequenced. Results One clone of human phage Fab monoclonal antibody against HIV 1 gp120 polypeptide was acquired. It has high affinity, specificity and inhibition rate and it belongs to IgG Ⅰ subclass and κ type. It's V h Η and V κ were derived from V h Ⅲ and V κⅢ.Conclusion The human phage Fab fragment against HIV 1 gp120 antigen site binding chemokine receptor was acquired.

关 键 词：人源抗HIV-1 gp120趋化蛋白受体结合位点Fab单克隆抗体基因 抗HIV-1噬菌体 Fab免疫球蛋白类 单克隆抗体 人类免疫缺陷病毒 艾滋病 AIDS 基因获得 

分 类 号：R392[医药卫生—免疫学]