B_(7-2)表达质粒对HBV DNA疫苗诱导的特异性免疫应答的影响  

Effect of B_(7-2) expression plasmid on the specific immune response induced by HBV DNA vaccine

作　　者：王延军[1] 靳清汉[2] 宋昌稳[2] 孙斌[3] 

机构地区：[1]山东省立医院中心实验室,济南250021 [2]山东金乡人民医院内科 [3]山东省立医院消化内科,济南250021

出　　处：《中华实验和临床病毒学杂志》2002年第4期370-372,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：山东省青年科学基金项目 (1999CA2DBB1)

摘　　要：目的　探讨B7 2 分子是否能够增强乙型肝炎病毒 (HBV)DNA疫苗诱导的特异性免疫应答。方法　将B7 2 表达质粒与HBVDNA疫苗共接种于小鼠腓肠肌内 ,检测细胞毒性T淋巴细胞 (CTL)活性 ,迟发性超敏反应 (DTH)及抗 HBs滴度。结果　B7 2 表达质粒与HBVDNA疫苗共接种组的DTH反应和CTL活性 ,明显强于单独接种HBVDNA疫苗组 (P <0 .0 1)。两组的抗 HBs滴度差异无显著性(P >0 .0 5 )。结论　B7 2 表达质粒与HBVDNA疫苗共接种可显著增强抗 HBV特异性细胞免疫应答(CMI)。Objective To determine whether B 7 2 could improve hepatitis B virus (HBV) specific immunity induced by inoculation of a plasmid encoding HBV preS2+S HBV DNA vaccine. Methods B 7 2 expression plasmids were inoculated with the DNA vaccine in the gastrocemius muscles. Then the cytolytic T Lymphocyte activity (CTL), the delayed type hypersensitivity (DTH) response and the titers of anti HBs were analysed. Results The DTH response and CTL activity were significantly enhanced when B 7 2 expression plasmids were coinoculated with the DNA vaccine ( P <0.01),but the titers of anti HBs were unaffected ( P >0.05). Conclusion The results suggest that B 7 2 expression plasmid could improve HBV specific cell mediated immunity induced by HBV DNA vaccine, and the gene based coinoculation strategy using HBV viral antigen and B 7 2 costimmulating could be a powerful means of combating HBV infection.

关 键 词：B7-2表达质粒 乙型肝炎病毒 DNA疫苗 特异性免疫应答 乙型肝炎 

分 类 号：R392[医药卫生—免疫学]