金钗石斛的茎段组织培养与植株再生  

Tissue culture of stem segment and plantlet regeneration of Dendrobium nobile

作　　者：高培元 陈健妙 甘铨 

机构地区：[1]海南热带植物组织培养研究中心,海南海口570216

出　　处：《中草药》2002年第11期1031-1033,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的　为了有效加速贵重药用植物金钗石斛的人工繁殖。方法　以带侧芽的金钗石斛嫩茎段作外殖体 ;不同种类的培养基、同种培养基用不同配比的激素进行培养。结果　以 MS培养基附加 6 - BA (0 .5 mg/ L )、NAA(0 .2 m g/ L)诱导分化最佳 ,附加 6 - BA (3.0 mg/ L)、NAA (0 .5 mg/ L)增殖最适 ,附加 IBA(0 .3mg/ L)、NAA(0 .1mg/ L )对生根最适 (生根率 95 % )。生根小苗在以椰渣、碎火山石块、碎砖块 (1∶ 1∶ 1)的基质中覆以干蕨长势良好。Object To speed up the artificial propagation of the costly medicinal plant Dendrobium nobile Lindl. Methods The tender stems with lateral buds were tried as the explants and cultured on various culture media with different portions of hormon. Results The best medium for bud induction was the MS basic medium with addition of 6 BA (0.5 mg/L), NAA (0.2 mg/L), while MS with the addition of 6 BA (3.0 mg/L) NAA (0.5 mg/L), was suitable for propagation, MS with addition of IBA (0.3 mg/L), NAA (0.1 mg/L) was better for root growing, the striking growth rate was up to 95%. The young sprout was cultured fine on the base material mixed with coco crumbs, volcanic rocks and brick fragments (1∶1∶ 1) and covered by dried pteridophyte. Conclusion To provide the effective ways to reserve the resources of D. nobile and last its utlization.

关 键 词：金钗石斛 组织培养 植物再生 人工栽培 茎段组织 

分 类 号：S567[农业科学—中草药栽培]