氟致大鼠胚胎发育毒性及其机理的体外实验研究  

Studies on the developmental toxicity of fluoride and its mechanism of rat embryos in vitro

作　　者：张本忠[1] 高小玲[2] 吴德生[3] 

机构地区：[1]兰州医学院公共卫生系,兰州730000 [2]甘肃省卫生厅检验中心 [3]华西医科大学环境卫生学教研室

出　　处：《卫生研究》2002年第6期420-422,共3页Journal of Hygiene Research

摘　　要：为探讨氟的发育毒性及机理 ,孕 9 5d大鼠胚胎于体外培养系统中给予不同剂量的氟化钠 ,培养48h后 ,观察胚胎生长发育和器官形态分化状况 ;应用DTNB直接法测定胚胎组织谷胱甘肽 (GSH)含量 ;以 1,6二苯己三烯为荧光探剂 ,用荧光偏振技术测定卵黄囊细胞膜脂质流动性。结果显示当培养液中 ,氟浓度为10 μg ml时 ,胚胎生长发育和分化明显被抑制 ;同时 ,畸形胚胎发生率明显升高 ,主要有前后神经管闭合不全 ,脑发育不良和体翻转不全等 (P <0 0 5 ) ;氟浓度为 10 μg ml时 ,胚胎组织GSH活性和卵黄囊细胞膜脂质流动性也显著降低 (P <0 0 5 )。上述效应均呈现出一定的剂量 -效应 (反应 )关系。提示氟有潜在的致畸性和胚胎毒性 。To investigate the developmental toxicity of fluoride and its mechanism, 9.5 day-old embryos of SD rat were explanted and cultured in a whole embryo culture system with fluoride concentrations from 2.5 to 30μg/ml for 48h. Each viable embryo was evaluated using Brown scoring system, and visceral yolk sac diameter, crown-rump and head length, and embryo dry weight were measured, as well as GSH content in embryonic tissue and membrane lipid fluidity of visceral yolk sac cell were determined by 5,5-dithion-bis-2-nitrobenzoic acid(DTNB), and DPH fluorescence polarization techniques respectively. Some reverse dose-dependent decreases in above mentioned parameters were observed. When fluoride doses were ≥10μg/ml, the embryonic growth and morphogenesis were inhibited significantly(P<0.05). GSH content in embryonic tissue and membrane lipid fluidity of visceral yolk sac cell reduced obviously at the concentration ≥10μg/ml(P<0.05). Otherwise, F-dose of 10μg/ml resulted in significantly elevating in the number of teratism including open neural tube, deficient in flexion(P<0.05). Fluoride could result in embryonic growth retardation and potential teratogenic toxicity. These effects might be due in part to decrease in GSH activity and membrane lipid fluidity.

关 键 词：氟 大鼠胚胎 发育毒性 体外实验 谷胱甘肽 卵黄囊 膜脂流动性 作用机理 

分 类 号：R995[医药卫生—毒理学]