一氧化氮拮抗肺成纤维细胞增殖的量效关系  

Dose-effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts

作　　者：杨振国[1] 王德文[1] 宋良文[1] 彭瑞云[1] 秦全红[1] 夏国伟[1] 李杨[1] 高亚兵[1] 崔雪梅[1] 

机构地区：[1]北京放射医学研究所,现单位解放军军需大学100850

出　　处：《中华放射医学与防护杂志》2002年第5期311-314,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 ( 2 980 72 13)

摘　　要：目的　应用硝普钠 (SNP)作为一氧化氮 (NO)供体观察NO对60 Coγ射线照射的人胚肺成纤维细胞 (HELF)增殖活力及其分泌功能的影响 ,以探讨NO的抗纤维化机理。方法　应用MTT比色法、Griess一氧化氮浓度测定法、免疫组化和原位杂交等方法检测成纤维细胞增殖活性及内皮素 (ET)、血管紧张素II(AII)和转化生长因子 β(TGFβ)的表达情况。 结果　低剂量的60 Coγ射线照射对人胚肺成纤维细胞有促增殖作用 ,以 5Gy剂量的促增殖作用最强 ;30h以内 ,硝普钠基本以线性方式释放NO ,平均增幅为 4 2 5μmol L ,初步认为 4～ 5μmol L的NO为抑制成纤维细胞增生的最佳浓度 ;未照射成纤维细胞不表达或仅微弱表达ET、AII和TGFβ ;照后细胞ET和TGFβmRNA及其蛋白表达显著增强 ,AII合成增加。结论　NO供体硝普钠可明显抑制成纤维细胞的过度增殖和分泌活动 ,随浓度的加大 ,抑制作用增强 。Objective\ To investigate the dose\|effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts. Methods\ MTT colorimetry and immunohistochemistry were used to observe the proliferative activity and expression of ET.TGFβ.AⅡ in fibroblasts. Results\ Low dose 60 Co γ\|ray promoted proliferation of cultured HELF cells especially 5 Gy.NO provider\|sodium nitroprusside released NO linearly within 30 hours by measuring the concentration of NO in the culture medium.The average increase amplitude was 4\^25 μmol/L.The optimum concertration to depress hyperplasia of fibroblast was 4～5 μmol/L.The ET、TGFmRNA transcription and protein expression of ET、TGF.AⅡ were increased after irradiation. Conclusion\ NO can inhibit excessive proliferation and secretion of fibroblasts in a dose\|dependent way.\;

关 键 词：一氧化氮 成纤维细胞 细胞增殖 量效关系 放射性肝纤维化 ^60Coγ射线 

分 类 号：R563[医药卫生—呼吸系统]