照射复合创伤愈合伤口成纤维细胞TGFβ1、TGFβR1蛋白及TGFβ1mRNA的表达与意义  

Expression of TGFβ1 and TGFβR1 in fibroblasts during healing of combined radiation wound

作　　者：秦全红[1] 王德文[1] 彭瑞云[1] 高亚兵[1] 谷庆阳[1] 夏国伟[1] 

机构地区：[1]北京放射医学研究所病理室

出　　处：《中华放射医学与防护杂志》2002年第5期330-333,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：全军九五重点招标课题资助项目(96Z0 0 8)

摘　　要：目的　探讨局部照射对创伤愈合过程中伤口 (Fb)TGFβ1及TGFβR1表达的影响及意义。方法　将 1 32只Wistar大鼠分为单纯创伤组 (对照组 )和照射复合创伤组 (照射组 ) ,用免疫组织化学、原位杂交和图像分析方法检测两组伤口愈合过程中伤后 1～ 60d各时间点伤口成纤维细胞中TGFβ1、TGFβR1蛋白及TGFβ1mRNA表达的变化规律。 结果　单纯创伤组伤口成纤维细胞TGFβ1、TGFβR1表达有明显的时相性 ,伤后 2～ 2 1d可见表达 ,1 0d为表达高峰 ;照射复合创伤组其表达减弱 ,且表达时相延长 ,伤后 2 8d仍有表达。结论　照射抑制伤口成纤维细胞内源性TGFβ1及TGFβR1的表达 。Objective\ In order to study the impact of local irradiation on expressions of TGF β1 and TGF β R1 in fibroblasts during wound healing. Methods\ Altogether 132 Wistar rats were divided into two groups:unirradiated wound group (control group, n = 66) and γ rays irradiated wound group (irradiation group, n =66).We investigated the expression patterns of TGF β1 and TGF β R1 in these two groups 1 to 60 days after injury by immunohistochemistry, in situ hybridization and image analysis. Results\ The expressions of TGFβ1 and TGFβ R1 in the control group had a temporal character,i.e.they were observed from 2 to 21 days after injury and the highest expression was on the 10 th day.In irradiation group,their expressions were weaker than those in the control group and the expression time was prolonged,i.e.,it was still observed on the 28 th day after injury. Conclusion\ Irradiation with γ rays can reduce the expressions of TGFβ1 and TGFβ R1 in wound healing fibroblasts,indicating that radiation inhibits the secretion of endogenous TGFβ1 and reduces the reactivity of wound to exogenous TGFβ1.\;

关 键 词：创伤愈合 成纤维细胞 TGFΒ1 TGFβR1 辐射损伤 动物实验 

分 类 号：R818[医药卫生—放射医学]