三种免疫学方法检测结核病可行性分析  

Analysis on 3 kinds of immunology methods combination for pulmonary tuberculosis case detection

作　　者：谢红歌[1] 张霞[1] 杜思和[1] 叶德普[1] 王国斌[1] 

机构地区：[1]河南省胸科医院中心实验室,河南郑州450003

出　　处：《河南医学研究》2002年第4期310-312,共3页Henan Medical Research

摘　　要：目的 :探讨三种免疫学方法联合检测肺结核病的诊断价值。方法 :采用金标法 (TB DOT)、PPDIgGELISA法(TB Ab)和一步免疫层析法 (TB CHECK)三种免疫学检测方法 ,对 3 4例菌阳肺结核、46例菌阴肺结核、3 0例非结核肺部疾病及 2 1例复强反应阴性健康人进行检测 ,并对其结果进行分析。结果 :单项检测的敏感性和特异性分别为TB DOT 88 2 %、99 2 % ;TB Ab 79 4%、98 1% ;TB CHECK 85 3 %、94 1%。单项检测菌阳和菌阴肺结核的阳性率分别为TB DOT 88 2 %、5 8 3 % ;TB Ab 79 4%、47 8% ;TB CHECK 85 3 %、45 6%。联合方法检测菌阳、菌阴肺结核阳性率二联分别为 91 2 %、65 9% ;三联分别为 94 1%、69 6% ,比单项检测明显提高 ,但特异性稍有降低。菌阳和菌阴肺结核联合检测 ,二联法同时阳性的阳性率分别为 85 3 %和 5 6 5 %。联合检测同时阳性比单独法检测低或相当 ,但特异性提高了 ,可达 98% ,若以健康对照组 ,可达 10 0 %。结论 :采用这三种联合检测方法 ,由于提高了特异性 ,且操作方法简便 ,经济实用 ,因此 。Objective: To evaluate the diagnostic effect of 3 kinds of methods for pulmonary tuberculosis.Methods: Examination results were analyzed in 34 cases of bacteriological positive,46 cases of bacteriological negative,30 cases of other lung disease,21 cases of health donor as a control (boost effect negative).DIGFA(TB DOT)?PPD IgG ELISA(TB Ab) and immunochromatography(TB CHECK) examination were taken.Results: In single examination group sensitivity and specificity of TB DOT?TB Ab?TB CHECK were 88 2%?92 2%;79 4%?98 1% and 85 3%?94 1% respectively.In combination examination all positive rate,2 methods combination was 85 3% for bacteriological positive cases;56 5% for bacteriological negative cases.All positive rate in combination examination group was obviously higher than that in single examination group.The specialty was 98% for healthy control and other lung disease group,100% for healthy control group.Conclusion: It can be widely expanded to detect pulmonary tuberculosis,because its simple operation and cheap price.

关 键 词：肺结核 金标法 免疫层析法 ELASA法 诊断 

分 类 号：R521[医药卫生—内科学] R446.61[医药卫生—临床医学]