血管内皮细胞与平滑肌细胞复合构建组织工程人工血管的实验研究  

EXPERIMENTAL STUDY OF TISSUE ENGINEERED BLOOD VESSEL WITH VASCULAR ENDOTHELIAL CELL AND VASCULAR SMOOTH MUSCLE CELL

作　　者：潘勇[1] 艾玉峰[1] 黄蔚[2] 赵玉峰[2] 熊猛[1] 张琳西[1,3] 

机构地区：[1]第四军医大学西京医院整形外科中心,西安710032 [2]第四军医大学西京医院眼科研究所 [3]第四军医大学生理教研室

出　　处：《中国修复重建外科杂志》2003年第1期65-68,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目 (30 0 70 777)

摘　　要：目的　构建既具有活性血管平滑肌细胞 (VSMC)中膜层 ,又有血管内皮细胞 (VEC)内皮化的复层结构组织工程血管支架材料。方法　 1将聚羟基乙酸 (PGA)纤维无纺网、VSMC和胶原纤维相混合 ,设计构建 VSMC活性的组织工程血管支架材料 ;2采用酶消化法从兔主动脉中分离培养兔 VEC并传代、纯化 ,接种于组织工程人工血管支架的内腔 ,体外培养 ,并行电镜观察。结果　构建的支架材料具有一定弹性和韧性 ,VEC在其内表面形成较完整内皮细胞层 ,且 VSMC能够保持活性 ,生长状况良好。结论　经胶原包埋处理的 PGA支架可作为组织工程化人工血管研究较理想支架材料 ;为组织工程方法构建具有分层结构的组织工程血管奠定实验基础。Objective To investigate the feasibility to seed vascular endothelial cell(VEC) and vascular smooth muscle cell (VSMC) into tissue engineered blood vessel scaffold material. Methods ①A blood vessel scaffold with a combined polymer was designed, which mainly is composed of rabbit VSMC and collagen with reinforcement by a non spinning fabric mesh made of polyglycolic acid (PGA). ② VEC were isolated from rabbit thoracic aorta by enzyme digestion methods and subcultured and purified. Then the cells were seeded into scaffold material. The morphological characteristics of tissue engineered blood vessel was analyzed by scanning electron microscopy. Results VEC could adhere well to the inner surface of the tissue engineered tubular scaffold material with a tenacity and elasticity. VSMC could sustain bioactivity of cell. Conclusion Non spinning PGA porous biodegradable materials coated with collagen is benefit for cells to adhere and grow. It will lay a foundation of a laminated structure of tissue engineered blood vessel.

关 键 词：血管内皮细胞 血管平滑肌细胞 组织工程 人工血管 聚羟基乙酸 

分 类 号：R318.11[医药卫生—生物医学工程]