从大口黑鲈胃、肠道中分离的部分好氧菌的研究  

STUDY ON THE STOMACHIC AND INTESTINAL PARTED OXYBIONTIC MICROFLORA OF MICROPTERUS SALMOIDES

作　　者：黄文芳[1] 庞启华[1] 李小波[1] 徐军[1] 符路娣[1] 吴小明[1] 

机构地区：[1]华南师范大学生命科学院,广东广州510631

出　　处：《华南师范大学学报（自然科学版）》2002年第4期10-14,共5页Journal of South China Normal University(Natural Science Edition)

摘　　要：从大口黑鲈（Micropterus　salmoides）胃、肠道中分离到 24个好氧菌株.经过自动微生物鉴定系统（VITEK-AMS-60和VITEK-AMS-CC2）鉴定结果是:真菌只有1个菌株（茁芽毛孢酵母Tri-chosporon　pullulans）;细菌有23个菌株,其中革兰氏阳性菌只有1个菌株（干燥棒状杆菌Corynebac-terium　xerosis）,1个非发酵性菌株（食酸假单胞菌Pseudomonasacidovorans）,其余21个菌株均是革兰氏阴性菌;革兰氏阴性菌中,支气管败血性鲍特氏菌（Bordetella bronchiseptica）5个,尿放线杆菌（Acti-nobacillus　ureae）4个,粘黄杆菌（Flavobacterium　gleum）4个,产吲哚黄杆菌（F.indologenes）3个,恶臭假单胞菌（P.putida）3个,泡囊假单胞菌（P.vesicularis）2个.24个菌株中5个水解淀粉,5个水解牛奶;在22种药物敏感试验中,恶臭假单胞菌肠14菌株和支气管败血性鲍特氏菌肠17菌株分别对17种药物有耐药性;24个菌株中,对氨苄青霉素、先锋V（头孢唑啉）、青霉素G不敏感的菌分别有23株、23株、22株.Twenty - four oxybiontic strains, including one fungal( Trichosporon pullulans) and 23 strains of bacteria, which have been identified by auto - microbiological identifical system (VUEK - AMS - 60 and VITEK - AMS - CC2) , were isolated from the stomach and intestine of the Micropterus salmoides. Among these bacterial strains, one was non - fermentation bacterial strain ( Pseudomonas acidavorans ) , one gram - positive strain ( Corynebacterium xerosis ) and twenty - one gram - negative strains which were Bordetella bronchiseptica (5 strains), Actinobaciilus ureae (4 strains ) , Flavobacterium gleum (4 strains ) , F. indologenes ( 3 strains) , P. putida (3 strains) , P. vesicularis (2 strains) . There were 5 strains to hydrolysis starch and 5 strains to hydrolysis milk. By testing of sensitivity to 22 drugs, P. putida (entl4 strain) and B. bronchiseptica (ent17 strain) weren't sensitive to 17 drugs. Ampi-cillin, cefazolin and penicillin G could not inhibit 23 strains, 23 strains and 22 strains, re-spectively.

关 键 词：好氧菌 大口黑鲈 胃肠道菌群 分离 菌株鉴定 药物敏感性 菌落形态 

分 类 号：Q93－331[生物学—微生物学]