乙酰唑胺对表达水孔蛋白1的非洲爪蟾卵母细胞转运水功能的影响  

EFFECT OF ACETAZOLAMIDE ON THE WATER TRANSPORTING FUNCTION OF XENOPUS OOCYTES EXPRESSING AQUAPORIN-1

作　　者：母生梅[1] 马兵[1] 姚小皓[1] 于和鸣 李学军[1] 

机构地区：[1]北京大学医学部基础医学院药理学系,北京100083 [2]国家计划生育委员会科学研究所,北京100081

出　　处：《药学学报》2002年第12期934-937,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金 ( 39770 2 86 ;39770 775 ;30 1710 90 ) ;美国洛克菲勒基金 (RF930 6 3#77;RF930 6 3# 82 )

摘　　要：目的　观察乙酰唑胺对水孔蛋白 1(AQP1)水转运功能的影响。方法　将AQP1cRNA 10ng注射入非洲爪蟾卵母细胞 ,观察并记录给予 1× 10 - 7～ 1× 10 - 5mol·L- 1 乙酰唑胺后卵母细胞在低渗溶液中的膨胀率 ,并计算其渗透水通透性 (Pf)的变化。结果　非洲爪蟾卵母细胞表达AQP1蛋白后 ,在低渗溶液中迅速膨胀 ,Pf值显著增加至 1 82×10 - 2 cm·s- 1 ,而注射水的阴性对照仅为 0 2 5× 10 - 2 cm·s- 1 。AQP抑制剂HgCl2 使Pf值降至 0 6 4× 10 - 2 cm·s- 1 ;1×10 - 7～ 1× 10 - 5mol·L- 1 乙酰唑胺处理后 ,Pf值分别降至 1 4 3,1 2 4和 0 93× 10 - 2 cm·s- 1 。结论　乙酰唑胺剂量依赖性地降低AQP1介导的渗透水通透性 ,抑制AQP1转运水的功能。AIM To observe the effect of acetazolamide on the water transporting function of Xenopus oocytes expressing aquaporin-1. METHODS Fifty nL AQP1 cRNA was injected into Xenopus oocytes, then the cells were transferred to low osmotic solution. The swelling of the cells in response to 1×10 -7～1×10 -5 mol·L -1 acetazolamide was recorded, and the corresponding osmotic water permeability (Pf) was calculated. RESULTS The Xenopus oocytes expressing AQP1 protein swelled immediately after transferred to low osmotic solution, with the Pf markedly increased to 1.83×10 -2 cm·s -1. While the Pf of negative group injected with ddH 2O remained as 0.25×10 -2 cm·s -1. Aquaporin inhibitor HgCl 2 decreased the Pf of AQP1 to 0.64×10 -2 cm·s -1; After treated with acetazolamide at the dose of 1×10 -7～1×10 -5 mol·L -1, the Pf decreased in a concentration-dependent manner. CONCLUSION Acetazolamide decreased the osmotic water permeability induced by AQP1 and inhibited the water transporting function of AQP1 in a dose dependent manner.

关 键 词：水孔蛋白1 水孔蛋白 乙酰唑胺 渗透水通透性 卵母细胞 

分 类 号：R965[医药卫生—药理学]