盘花垂头菊中两种新型倍半萜类化合物抑制人肝癌SMMC-7721细胞生长  

INHIBITION OF NOVEL HIGHLY OXYGENATED BISABOLANCE SESQUITERPENE COMPOUNDS FROM CREMANTHODIUM DISCOIDEUM ON THE GROWTH OF HUMAN HEPATOMA CELL LINE SMMC-7721

作　　者：张琪[1] 王勤[1] 苗瑞东[1] 王芳[1] 

机构地区：[1]兰州大学生命科学学院,甘肃兰州730000

出　　处：《药学学报》2002年第12期993-995,共3页Acta Pharmaceutica Sinica

基　　金：甘肃省科技攻关项目 (GS0 0 3 A5 2 0 2 2 )

摘　　要：AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTS The IC 50 of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL -1 and (22±9) μg·mL -1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL -1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSION The two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTS The IC 50 of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL -1 and (22±9) μg·mL -1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL -1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSION The two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.

关 键 词：盘花垂头菊 甜没药烯型倍半萜化合物 SMMC-7721细胞 DNA损伤 细胞凋亡 药理学 肝癌 

分 类 号：R273.57[医药卫生—中西医结合] R285[医药卫生—中医肿瘤科]