载α-干扰素的聚-L-乳酸片层粒子的制备研究  

Preparation of Interferon-α-Loaded Poly-L-lactic Acid Lamellar Particles

作　　者：李雄伟[1] 陈晓理[2] 刘佳林[1] 曹伟民[1] 

机构地区：[1]中国科学院成都有机化学研究所,成都610041 [2]四川大学华西医院肝胆胰研究室,成都610041

出　　处：《生物医学工程学杂志》2002年第4期559-562,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 ( 5 98730 2 5 );中国科学院留回国人员择优资助项目

摘　　要：采用结晶性聚 - L-乳酸 ( PL L A)以非溶剂沉淀技术制得粒径均匀的片层粒子 (简称片粒 ) ,其粒径为 3～5 μm,呈菱形或阶梯状不规则外形。PL L A片粒在无任何表面活性剂、分散剂的条件下制得 ,表面清洁 ,不含有任何杂质 ,十分有利于以吸附方式携载蛋白 ,从而避开有害的有机溶剂环境及机械搅拌 ,有效地保护蛋白分子。以 α-干扰素 ( IFN- α)为模型蛋白 ,发现当 IFN与 PL L A片粒的质量比小于 1%时 ,IFN的吸附效率可超过 95 %。所携载IFN的释放可延续 10 d以上 ,释放的 IFN完好地保留了原有的生物活性。PL L A片粒具有易被巨噬细胞吞噬的特性 ,该特性对于激活巨噬细胞的免疫功能 。Lamellar particles( lamellae) were prepared by non-solvent precipitation from crystalic poly-L-lactic acid (PLLA). The PLLA lamellae exhibite a diamond or stepped irregular shape with a size range between 3~5μm. Prepared without any surfactants and dispersing agents, the lamellar particles have clean surface, which is advantageous for the adsorption of proteins. The PLLA lamellar particles adsorbed protein cytokine interferon-α(IFN-α) with an adsorption efficiency of ≥95%. The release of loaded IFN could continue for more than 10 days. The cell incubation experiments showed that the PLLA lamellar particles were easy to be phagocytosed by macrophages. The immunological experiments showed that the biological activity of IFN-α loaded on the PLLA lamellar particle was effectively retained.

关 键 词：聚-L-乳酸 片层粒子 Α-干扰素 吸附 生物活性 药物载体 

分 类 号：R318.08[医药卫生—生物医学工程]