检测丙型肝炎核心抗体的合成肽酶联免疫试验  

The development of an enzyme-linked immunoassay for detection of antibody to hepatitis C virus core antigen

作　　者：杨守昌[1] 梁炽森[1] 周荣[1] 骆抗先[1] 

机构地区：[1]南方医院传染病科

出　　处：《第一军医大学学报》1992年第1期14-16,共3页Journal of First Military Medical University

基　　金：总后卫生部"八五"攻关课题

摘　　要：为建立一种以酶联免疫试验检测丙型肝炎病毒的核心抗体,按病毒氨基酸序列C区合成一个36-寡肽,将其作为试剂抗原包被固相,以捅获待检血清的相应抗体,以酶标抗入IgG将其检出。输血后非甲非乙肝炎32例检出抗体20例(62.5％),其中发病1个月内的13例中检出8例。特异性由抑制试验确定。17例阳性血清可用聚合酶链反应检出病毒。结果表明,完善这一系统可能发展为试剂盒。To develop an enzyme-linked immunoassay for detection of antibody to hepatitis C virus core antigen, a 36-amino acid oligopeptide was synthesized. This synthetic peptide was used as the antigen coated on the solid phase to capture the corresponding antibody in patient's serum, then the antibody was detected with the enzyme-labelled anti-human IgG/γ The antibody was detected in 20 of 32(62.5%) patients with transfusion hepatitis non-A, non-B,and eight of 13 cases were detected within one month after onset of the disease. The specificity was confirmed by the inhibition test. The virus RNA was also detected in 17 of the 20 antibody positive cases. The results revealed that a test kit might be developed after improve-ment and consummation.

关 键 词：丙型肝炎 核心抗体 合成肽 ELISA 

分 类 号：R512.603[医药卫生—内科学]