空气卫生指示菌——涎链球菌的一种新型鉴别选择性培养基的研制及其应用  

Study of a new selective medium for the recovery of the air hygienic indicator-airborne Streptococcus salivarius

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作  者:余清[1] 戴景林[2] 

机构地区:[1]第一军医大学微生物学教研室 [2]军事医学科学院生物工程研究所

出  处:《第一军医大学学报》1992年第3期228-234,共7页Journal of First Military Medical University

摘  要:我们在S1培基的基础上改进研制了一种鉴别选择性较好,经济简便。适于推广的NSS培基。经实验室及现场采样考核,NSS能有效地抑制空气中杂菌的生长,采集的涎链球菌能形成特征性菌落,经染色镜检及生化鉴定,88.3%证实为涎链球菌。用NSS培基初步测定了几个场所空气中的涎链球菌含量:某市永定路商场空气中平均为5977.33CFU/m^3;幼儿园上图画课时为106.67CFU/m^3,上唱歌课时为400.00CFU/m^3。商场涎链球菌气溶胶粒谱测定结果:计数中值空气动力学直径为4.82μm,几何标准差为1.78,粒径在5.0μm以下的占52%,大于和等于7.0μm的占31.08%,表明空气中的涎链球菌含量与呼吸道活动密切相关。此项研究为空气卫生指示菌的检测和空气微生物卫生指标的研究提供了部分依据。Up to now, there is only one selective medium for the airborne Strep.saliva-rius so far as we know,which was developped by williams and Hirch and is called S1. According to the results of our tests, the inhibitory activity of this medium is too strong for the growth of Strep, salivarius.Besides, it needs a relatively high content of normal horse serum, which is rather expensive, difficult to obtain and causes the preparation as well as preservation troublesome.All of these will hinder the medium to be used in fields for extensive investigation. So we developped a new economic, simple and selective medium called NSS.It is demonstrated with our experiments in laboratory and on the fields that this new medium can inhibit the growth of most miscellaneous airborne bacteria, including moulds, collected from the atmosphere, and on this medium the collected Strep, salivarius can characteristic colonies distingushable from those of other organisms. The agreement rate of the presumative Strep, salivarius primarily selected according to the characteristic colonies with the standard strain of Strep, salivarius is 88.3% as verified by microscopic and biochemical tests. This study may offer a part of the technical basis for studying air hygienic indicator of respiratory contagious diseases.

关 键 词:涎链球菌 空气 卫生指示菌 

分 类 号:X830.5[环境科学与工程—环境工程]

 

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