间接ELISA检测羊伪狂犬病抗体的研究  

Indirect ELISA for detection of antibody to PRV inactirated vaccine

作　　者：崔保安[1] 杨明凡[1] 李瑞芳[1] 张素梅[1] 王学斌[1] 王莉[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《畜牧与兽医》2003年第1期10-12,共3页Animal Husbandry & Veterinary Medicine

摘　　要：利用差速离心纯化MDBK细胞增殖伪狂犬病毒 (PRV)为抗原 ,建立间接ELISA检测伪狂犬病抗体方法。选用抗原最佳包被浓度为 1∶80 0(4μg/mL) ,酶标兔抗山羊IgG最佳稀释浓度为 1∶80 0 ,作用时间为 60min,封闭物采用 4 %明胶 ,抗原抗体最佳作用时间为 60min。根据建立的最佳反应条件检测伪狂犬病毒油乳剂灭活苗、氢氧化铝凝胶灭活苗、基因缺失油乳剂灭活苗接种山羊后的抗体消长规律。试验表明间接ELISA检测PRV抗体具有快速、敏感、特异等特点 ,适合于大量样品的检测 。An indirect ELISA was established for detection of antibody against PRV in goat sera, employing the antigen which was the sediment through different centrifugal speed and was used to monitor the antibody dynamic regularity of goats after being immunited with inactivated vaccine. The well of an ELISA plate were coated with 0 4 μg antigen. The working titer of conjugate was 1∶800 dilution and the working time is 60 min. 4% was chosen for blocking agent. The time for antigen and antibody inter working was chosen to be 60 min. The result showed that indirect ELISA appeared correspondence in its specificity, sensitivity and repeatability test, and relatively practical for antibody surveillance of PRV.

关 键 词：间接ELISA 羊 伪狂犬病 抗体 

分 类 号：S858.26[农业科学—临床兽医学]