GNA和α-PAP双抗表达载体的构建及对烟草的遗传转化  

Construction of binary expressing vector containing GNA gene andα-PAP gene and transformation of the vector into Nicotiana tabacum

作　　者：柴红梅[1] 张绍松[1] 万萌[1] 赵永昌[1] 黄兴奇[1] 

机构地区：[1]云南省农业科学院生物技术研究所,云南昆明650223

出　　处：《西南农业学报》2002年第4期35-37,共3页Southwest China Journal of Agricultural Sciences

基　　金：云南省应用基础研究(97C001G)

摘　　要：将带有启动子GaMV35S、终止子Nos ter的雪花莲凝集素基因GNA插入到已构建好的植物表达载体pBI121/α PAP上,构建成双抗表达载体pBI121/GNA+α PAP,并采用农杆菌介导法将其转化进入烟草红花大金元,获得了卡那霉素筛选的抗性植株,通过PCR扩增验证,烟草基因组中含有这两个抗性基因。A plant expression vector with resistance to both virus and aphid was constructed by inserting Galanthus nivalis agglutinin gene(GNA)containing CaMV35S promoter and Nos terminal into pHI121／αPAP,a Pokeweed Antiviral Protein gene.GNA gene and αPAP gene were transferred into tobacco mediated by Agrobacterium tumefaciens and under Kanamycin selection pressure.The results of PCR from the kanamycin resistant transformants indicated that GAN gene and αPAP gene have been integrated into the tobacco genome.

关 键 词：GNA α-PAP 双抗表达载体 烟草 遗传转化 雪花莲凝集素 商陆抗病毒蛋白 表达载体构建 

分 类 号：S572[农业科学—烟草工业]