农杆菌对中华猕猴桃叶柄的遗传转化初报  

Transformation of Actinidia chinensis with Agro-bacterium tumefaciens

作　　者：黄萍[1] 沈孝善[1] 马朝宏[1] 

机构地区：[1]贵州省农业科学院生物技术研究所,贵州贵阳550006

出　　处：《西南农业学报》2002年第4期113-115,共3页Southwest China Journal of Agricultural Sciences

摘　　要：以中华猕猴桃79 2的叶柄为受体,根癌农杆菌(Agrobacteriumtumefaciens)IBA4404(pBI121)作介导菌,将叶柄与农杆菌共培后转移到含25μg/mL卡那霉素(Kan)的筛选培养基上筛选,初步获得了抗Kan的抗性苗。The genetic transformation was conducted using Agrobacterium tumefaciens IBA4004(pBI 121)as the vector and the leaf stalk of Actinidia chinesis cv.792 as the acceptor.Then the leaf stalks of 792 were transferred onto the medium containing 25 μg/mL Kanamycin after cocultured with A.tumefaciens.Some Kanresistant plantlets were screened.

关 键 词：农杆菌 中华猕猴桃 叶柄 遗传转化 

分 类 号：S663.4[农业科学—果树学]