从单头灰飞虱中检测水稻黑条矮缩病毒简单快速的方法  

Rapid methods for the detection of rice black streaked dwarf Fijivirus in a single planthopper

作　　者：王朝辉[1] 周益军[1] 范永坚[1] 程兆榜[1] 张文荟[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《上海交通大学学报（农业科学版）》2002年第4期340-343,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：江苏省"九五"重点攻关 (BE963 86);江苏省自然科学基金 (BJ9713 2 )

摘　　要：报道了 2种从介体灰飞虱中检测水稻黑条矮缩病毒的分子生物学方法。其中直接捕获 RT-PCR经简单的样品处理后可以从单头虫粗提液的 1 0 - 3中检测到病毒 ,并具有快速、简单优点 ;单头灰飞虱样品汁液点膜或虫体直接积压在膜上杂交检测具有简单、经济和快速的特点 ,可以检测田间批量样品 ,用于病害流行研究和测报。研究中 60 % -70 %杂交检测阳性的介体生物学接种结果为阴性 。Two methods based on molecular biology for the detection of rice black streaked dwarf Fijivirus(RBSDV) have been established. Of which direct binding RT PCR is fast, simple and sensitive that can detect the virus in 10 3 dilution of the crude sap of a single planthopper Laudelphax striatellus (Fallén). For hybridization detection the membrane can both blotted with planthopper sap and squashed insect material. This method is simple, fast and economic and can be applied for the study of epidemiology and prediction of diseases caused by RBSDV. Comparison of the detection by hybridization and biological method indicate 60% 70% viruliferouse planthopper can not inoculate the maize seedling under given conditions.

关 键 词：单头灰飞虱 水稻 黑条矮缩病毒 RT-PCR 杂交 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]