维生素E、C和HUFA交互作用对中华绒螯蟹生殖性能的影响  

Effect of vitamin E and vitamin C, highly unsaturated fatty acids,in broodstock diet on reproduction performance of Eriocheir sinensis

作　　者：艾春香[1] 陈立侨[1] 温小波[1] 周忠良[1] 王群[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《水产学报》2002年第6期533-541,共9页Journal of Fisheries of China

基　　金：上海市"曙光计划"基金项目(975G06);高等学校博士点专项基金项目(2000026903)

摘　　要：通过投喂添加或不添加VE、VC和HUFA的四组实验饲料,经198d饲养实验,以雌蟹的性腺系数、产卵力、孵化率、各组织超氧化物歧化酶(SOD)活性和过氧化产物丙二醛(MDA)含量等为指标,研究这两类营养素交互作用对其生殖性能的影响。结果表明,VE、VC和HUFA对雌蟹生殖性能有显著的影响(P<0.05):在产卵力方面,1组为3790个卵细胞·g-1体重,极显著高于2组(为2317个卵细胞·g-1体重)和3组(为2129个卵细胞·g-1体重)(P<0.01),显著高于4组(为3050个卵细胞·g-1体重)和5组(对照组)(为3010个卵细胞·g-1体重)(P<0.05);而孵化率方面,1组(86.17%)极显著高于2组(36.06%)、3组(29.18%)和4组(25.73%)(P<0.01),也显著高于5组(71.12%)(P<0.05)。1组和2组雌蟹性腺中SOD活性分别为40.09Nu·mL-1和39.87Nu·mL-1,相应地,MDA含量分别为6.87nmol·mL-1和3.33nmol·mL-1;而3组和4组雌蟹,尽管其性腺中SOD活性较高,分别为79.21Nu·mL-1和85.2Nu·mL-1,但由于饲料中抗氧化性VE、VC的缺乏,导致脂质过氧化发生,其MDA含量显著增加(P<0.05),分别为12.65nmol·mL-1和25.18nmol·mL-1。4组雌蟹的产卵力较大,但因VE、VC的缺乏发生脂质过氧化,导致所产的卵质低下,从而影响了卵子的孵化率。结果表明,VE。198day feeding trials were conducted to determine the reciprocal physiological effects on Chinese mittenhanded crabs Eriocheir sinensis female broodstock by feeding with four experiment diets and one control group diet for: diet 1 supplementing HUFA and vitamin E, C; diet 2 supplementing only vitamin E and C; diet 3 supplementing neither HUFA nor vitamin E and C; diet 4 supplementing only HUFA, diet 5 (control group diet) was fresh clam. The results showed that vitamin E, vitamin C and HUFA were essential components in broodstock diets. Fecundity and hatchability of eggs from broodstock crabs fed with diet 1 in which HUFA and vitamin E, C were supplemented were 3790 egg cell·g-1 and 86.17% respectively, were the very significantly higher than those of broodstock crabs fed with HUFAdeficient diet like diet 2 and 3 (2317egg cell·g-1, 36.06% and 2129egg cell·g-1, 29.18%) (P<0.01), and significantly higher than those of and fecundity and hatchability of eggs (3050 egg cell·g-1 and 25.73% in diet 4 treatment) from the crabs fed with diet 4 were also lower than those of control group (3010 egg cell·g-1 and 71.12% respectively) (P<0.05). When the broodstock crabs were fed with vitamin E and Cdeficient diets like diet 3 and 4, lipids of crabs were peroxidized with decreasing SOD activity and increasing MDA content. Meanwhile, the effect of total lipid content, fatty acid composition and vitamin E and C concentrations of experimental diets on those of corresponding eggs, and the lipid peroxidation in eggs are discussed. The results of this study indicate both HUFA and vitamin E, C are indispensable and cooperative to egg developing of crab and must be supplemented in the broodstock crab diet.

关 键 词：中华绒螯蟹 维生素E 高不饱和脂肪酸 交互作用 生殖性能 维生素C 

分 类 号：Q959.223.5[生物学—动物学] S[农业科学]