植物生长调节剂对石竹试管成花及内源激素与多胺的影响  

Effects of Plant Growth Regulators on Flowering and Changes of Contents of Endogenous Hormones and Polyamines During Flowering of Dianthus chinensis L.

作　　者：桂仁意[1] 曹福亮[1] 沈惠娟[1] 谢寅峰[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2003年第1期6-10,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金资助项目(29770604)

摘　　要：以无根石竹试管苗为试材,用植物生长调节剂调控石竹试管成花,同时测定了成花过程中内源激素与多胺的变化。结果表明,低浓度NAA有利于成花,0.2mg/L的NAA可将成花率由对照的33.3%提高到71.7%;6 BA抑制成花,浓度为0.5mg/L时便完全抑制成花。在成花过程中,iPAs,ZRs,IAA,Spd含量出现峰值,且含量的增加有利于成花。IAA/(iPAs+ZRs)的比值影响石竹试管苗的发育,当这一比值较低时,石竹便一直处于营养生长状态。This paper intends to find out the effects of plant growth regulators on flowering and the metabolism of hormones and polyamines during flowing of Dianthus chinensis L.Small pieces of young leaves of Dianthus chinensis L.collected from the campus of Nanjing Forestry University in spring were cultured on the dedifferentiation medium of MS+6BA 2.0 mg/L+2,4D 0.2 mg/L after sterilization.Green yellow calli were induced from the explants after one week of culture and then were subcultured on the differentiation medium of MS+KT 0.3 mg/L+6BA 0.3 mg/L+NAA 0.1 mg/L.Four weeks later,shoots appeared in some culture bottles.4～5 cm long shoots were cut at bottom and the same amount of shoots(60) were cultured on control MS medium and treat MS media supplemented with 6BA or NAA with serial concentration of 0.05 mg/L,0.2 mg/L,0.5 mg/L,1.0 mg/L.The experiments were repeated 4 times.Flower buds appeared on some media two weeks after inoculation and then flowered 4～5 days later.The apexes were collected every 5 days from the beginning day of culture for detecting the contents of zeatin tiboside group(ZRs),isopentenyl adenine group(iPAs) indole3acetic acid(IAA),gibberellin acid(GA1/3),abscisic acid(ABA),putresine(Put),spermidine(Spd),spermine(Spm) and diaminopropane(Dap).The culture results showed that 6BA inhibited flowering:0.1 mg/L 6BA decreased the flowering rate from 33.3% to 20% significantly and 0.5 mg/L 6BA inhibited flowering completely.0.1 mg/L and 0.2 mg/L NAA enhanced the flowering rate significantly to 56.7% and 71.7% respectively while 0.5 mg/L NAA decreased the flowering rate significantly to 21.7% and 1.0 mg/L NAA inhitited flowering entirely,which indicated that NAA promoted flowering with low concentration and inhibited flowering with high concentration.Endogenous iPAs,ZRs,IAA had their peak contents during flowering,and their higher contents were more favorable to flowering.But the development was affected by the ratio of IAA/(iPAs+ZRs),and when the ratio was low,�

关 键 词：植物生长调节剂 石竹 多胺 成花 试管苗 内源激素 

分 类 号：S681.5[农业科学—观赏园艺] Q945.64[农业科学—园艺学]