XeCl准分子激光辐照生物大分子对其结构影响初步研究——Ⅰ308nm紫外激光辐照小牛胸腺 DNA的电镜观察与分析  

Study of the Effect of XeCl Excimer Laser Radiation on Biomacromolecule Structure BTG DNA

作　　者：黄汝多[1] 葛继志[1] 李振华[1] 朱峰[1] 陈涛[1] 智小勇[1] 陈永荣[2] 殷宣民 

机构地区：[1]安徽大学生命科学学院,中国安徽合肥230039 [2]中国科学院安徽光机所,中国安徽合肥230031

出　　处：《激光生物学报》2002年第6期423-426,共4页Acta Laser Biology Sinica

摘　　要：XeCl308nm紫外激光(单脉冲输出能量33.4mJ,脉冲频率2次/秒,辐照时间45秒,光斑6×3mm,透镜焦距300mm)辐照330mm处小牛胸腺DNA(固体)。用扫描电子显微镜和透射电子显微镜可以观察到DNA受照射后有断裂、分叉、扭曲、聚集和交联等现象,影响生物大分子的初级结构和高级结构的变化。在我们的实验条件下是以影响DNA构象变化为主。The biomacromolecule of Bovine Thymus Gland DNA(BTG DNA) poduter ,was radiated by XeCl excimer laser (308 nm) in the condition of export out power 33.4mJ/per pulse,pulse frequency 2 times/sec.,and radiation time 45sec. And in the conditions of the sample DNA was set on 330 mm far from laser source. The sample DNA radiated and its control were observed and measured by scanning electron microscope and perspective electron microscope. And then compared the data between two. The results showed that, when the BTG DNA was radiated by XeCl excimer laser, either its primary structure or its conformation expressed changes in breakage of DNA chains, or expansion, branching, sprain wrench, accumulation, intersect, and interlock of DNA chains. Especially it induced the BTG DNA conformation to change in our experiment condition. 

关 键 词：XECL准分子激光 辐照 生物大分子 结构影响 紫外激光 小牛胸腺 DNA 电镜观察 

分 类 号：Q631[生物学—生物物理学]