半乳糖氧化酶的生物电化学特性  

Bioel ectrochemical Characterization of Galactose Oxidase(GAO)

作　　者：贾能勤[1] 朱燕[1] 江丽萍[1] 吴霞琴[1] 章宗穰[1] 

机构地区：[1]上海师范大学生命与环境学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2002年第4期38-41,共4页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市教委发展基金(CL9709);上海师范大学科研基金资助项目(DQ08)

摘　　要：采用循环伏安法对半乳糖氧化酶(GAO)进行了生物电化学特性的研究。实验结果表明,由于半乳糖氧化酶的酶活性中心深埋在酶内部,所以该酶在铂电极上的直接电化学行为较难观察到,而二茂铁甲醇作为介体能明显地改善酶活性中心的电子传递,加快电子传递速率。且测定了半乳糖氧化酶与二茂铁甲醇之间的反应速度常数Ks为1.2×10~5L·mol^(-1)·s^(-1)。The bioelectrochemical characterization of Galactose oxidase was studied by cyclic voltammetry. Direct cyclic voltammgram of GAO showed the irreversible behavior on the electrode, which meaned electron transfer was very slow due to the activation center deeping into GAO enzyme. Ferrocene methanol, as a mediator, could speed up the electron transfer from activation center of enzyme to the surface of the electrode. Furthermore, reaction rate con-stant(Ks=1. 2x105L-mol-1s-1) was determined between GAO and Ferrocene methanol.

关 键 词：生物电化学特性 半乳糖氧化酶 二茂铁甲醇 循环伏安法 酶活性 电子传递 

分 类 号：Q554[生物学—生物化学]