反相高效液相色谱法测定三白草中槲皮素和芦丁的含量  

Determination of quercetin and rutin in Saururus Chinensis (Lour) Baill by RP-HPLC

作　　者：许雪琴[1] 叶蕻芝[2] 齐秀贞[1] 陈国南[1] 

机构地区：[1]福州大学化学化工学院,福建福州350002 [2]福建中医学院中心实验室,福建福州350003

出　　处：《福州大学学报（自然科学版）》2002年第6期870-872,共3页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省教育厅科研项目 (JB0 2 0 6 3)

摘　　要：采用反相高效液相色谱法 (RP -HPLC)在C1 8色谱柱上以甲醇 :0 .2 %磷酸溶液 (5 0∶5 0 )为流动相测定了三白草中槲皮素和芦丁的含量 .流动相的流速为 1mL min ,检测波长 370nm .结果表明槲皮素在 0 .0 0 8-0 0 84 μg、芦丁在 0 .0 5 4 - 0 .5 4 0 μg范围内呈良好的线性关系 ,相关系数分别为 0 .9988和 0 .9999.槲皮素回收率为 99% ,RSD为 2 .0 % (n =5 ) ,芦丁为 10 6 % ,RSD为 1.4 % (n =5 ) .A method for determining quercetin and rutin in Saururus Chinensis (Lour.) Baill by RP-HPLC was described. The determination was carried out over a C 18 column at room temperature. Methanol: 0.2% phosphoric acid solution (50∶50) was used as mobile phase at a flow of 1?mL/min and detection wavelength was set at 370?nm. The calibration curves were linear in the range of 0.008-0.084?μg for quercetin (R=0.998?8) and 0.054-0.540?μg for rutin respectively. The recovery of quercetin was 99% (RSD=2.0%, n=5) and of rutin was 106% (RSD=1.4%, n=5) respectively.

关 键 词：反相高效液相色谱法 三白草 槲皮素 芦丁 含量测定 中草药 

分 类 号：R284.1[医药卫生—中药学] Q946.[医药卫生—中医学]