细胞周期调控蛋白在人类肾小球肾炎中肾小管及间质细胞的表达及其意义  

Expression of cell cycle regulatory proteins in renal tubulointerstitial cells of human glomerulo- ncphritis

作　　者：曾瑜[1] 魏仁桂[1] 李易娟[1] 陈述枚[1] 莫樱[1] 林穗珍[1] 许韩师[2] 

机构地区：[1]中山大学附属第一医院儿科,广州510080 [2]中山大学附属第一医院风湿科,广州510080

出　　处：《中华肾脏病杂志》2002年第6期408-412,共5页Chinese Journal of Nephrology

摘　　要：目的 观察人类肾小球肾炎时肾小管-间质细胞的细胞周期调控蛋白的表达情况。方法 采用免疫组织化学技术,检测19例肾小球肾炎患者肾穿刺标本中细胞周期正性调控蛋白周期素D1(cyclin D1)、周期素A(cyclin A)、细胞周期负性调控蛋白p21CIPI(p21)和增殖细胞核抗原(PCNA)的表达。结果 在人类肾小球肾炎中肾小管上皮细胞及间质细胞均见cyclin D1、cyclin A及p21的表达,并与PCNA呈正相关。小管的阳性表达以间质病变Ⅰ级和Ⅱ级组显著,间质阳性细胞数与小管间质病变程度及患者尿NAG活性呈显著正相关。结论 人类肾小球肾炎时,细胞周期调控蛋白参与肾小管上皮细胞及间质细胞的增殖,参与肾间质纤维化的发展。Objective To examine the expression of cell cycle regulatory protiens in renal tubulointerstitial cells of human glomerulonephritis. Methods Immunohisochemieal studies were performed on 19 specimen from renal biopsy to detect cyclin Dl, cyclin A, p21 and proliferating nuclear antigen (PCNA) . Results Cyclin Dl, cyclin A and p21 were positive in some of tubulointerstitial cells, and showed significant correlations with positive PCNA cells. The numbers of tubular positive cells in both groupsofⅠand Ⅱ degree of histopathological change were more than those of other groups. The numbers of interstitialpositive cells showed significant correlations with the degree of tubulointerstitial histopathological change and the value of urine NAG. Conclusion Cell cycle regulatory proteins regulate the proliferation of tubular and interstitial cells, and correlate with the interstitial fibrosis.

关 键 词：细胞周期调控蛋白 肾小球肾炎 肾小管 间质细胞 表达 免疫组织化学 

分 类 号：R692.3[医药卫生—泌尿科学]