低切应力对体外培养动脉的平滑肌细胞增殖和凋亡的影响  

EFFECTS OF LOW SHEAR STRESS ON PROLIFERATION AND APOPTOSIS OF VASCULAR SMOOTH MUSCLE CELLS OF ORGAN-CULTURED ARTERY IN VITRO

作　　者：刘艳春[1] 姜宗来[2] 刘波[1] 张峰[1] 张炎[1] 李玉泉[1] 

机构地区：[1]第二军医大学解剖学教研室,上海200433 [2]上海交通大学医学院,上海200030

出　　处：《医用生物力学》2002年第4期198-202,W003,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金资助项目 ( 10 13 2 0 2 0 ;10 0 72 0 75 );国家重点基础研究发展规划 ( 973 )资助项目 (G2 0 0 0 0 5 69- 0 5 )

摘　　要：目的　研究切应力对完整血管的生物学作用。方法　应用一种新的血管体外应力培养系统 ,控制定常流和压力 1 0 0mmHg,在低切应力条件下体外培养猪颈总动脉 1、4、7d采用BrdU免疫组织化学、TUNEL法、Hoechst 3 3 2 5 8荧光染色、透射电镜等方法观察血管平滑肌细胞的增殖和凋亡情况。结果　低切应力作用下血管平滑肌细胞的增殖率呈逐渐上升的趋势 ,而凋亡率则先上升后下降 ,其中凋亡率在低切应力作用 1d时达到高峰。结论　在低切应力条件下血管发生重建 ,血管平滑肌细胞在增殖的同时还存在凋亡 。Objective To explore the biological influence of shear stress on intact artery. Methods The common carotid arteries of pigs were cultured with a new artery organ-culture system under shear stress in vitro. 100mmHg of pressure and stable flow were selected. After 1, 4 and 7 day, immunohistochemistry was performed on the sections of artery with antibody to antigen BrdU. Apoptosis were identified by morphology, TUNEL and Hoechst 33258 staining. Results The results showed that the proliferation rate of vascular smooth muscle cells (VSMC) increased continuely while the apoptosis rate increased at first and then decreased under low shear stress, maximum on day 1. Conclusion These results suggest that proliferation and apoptosis of VSMC have important effects on vascular remodeling under low shear stress; Unbalance of proliferation and apoptosis may be one of foundations of cardiovascular diseases.

关 键 词：切应力 器官培养 血管重建 平滑肌细胞 细胞增殖 细胞凋亡 

分 类 号：R318.01[医药卫生—生物医学工程]