兔背根神经节植块法的雪旺氏细胞培养  

Culture of Schwann cells from dorsal root ganglion of newborn rabbits in vitro

作　　者：杨浩[1] 游思维[1] 王春婷[1] 吴玉梅[1] 李静雯[1] 鞠躬[1] 

机构地区：[1]第四军医大学神经科学研究所细胞生物学研究室,西安710032

出　　处：《解剖学杂志》2002年第6期513-516,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30070799)

摘　　要：目的:建立一种新的培养雪旺氏细胞(Schwann cell,SC)的方法,为神经移植研究获取高纯度的细胞奠定基础。方法:采用植块培养的方法,分离新生1～2天兔子的背根神经节(Dorsal root ganglion,DRG),弃膜,剪碎进行植块的雪旺氏细胞的培养,然后采用阿糖胞苷(cytosine arabinoside,Ara-C)抑制,差速贴壁法、Forskolin的牛垂体提取液(bovine pituitary extract,BPE)刺激进行细胞纯化,最后S-100免疫组织化学染色进行纯度鉴定。结果:本实验采用背根神经节植块及纯化的方法获得雪旺氏细胞的纯度可达95％以上。结论:这种选用DRG组织块培养及纯化SC的方法效率很高而且简单方便,所获SC的纯度高数量大。Objective:To establish a novel method for Schwann cell (SC) culture and purification in vitro. Methods primarily cultured SC were dissociated from the dorsal root ganglions (DRGs) of newborn rabbits. Briefly,DRGs were obtained from 1 to 2-day-old-rabbits. The pia of DRGs were cleared,and DRGs were cut into tiny pieces, then explanted and cultured. The purification of cultured SC were undertaken by adding cytosine arabinoside into cultures followed by differential adhesion method. Forskolin and bovine pituitary extract (BPE) were used to stimulate the SC to divide and enrich. The purity of the SC was evaluated according to the percentage of S-100-immunostaining cells. Results:With the culturing method used in the presented study,highly enriched population of SC reached a percentage higher than 95% . Conclusion: The present method is simple, convenient and highly effective for the SC purification, the SC is high in purity and large in quantity.

关 键 词：雪旺氏细胞 背根神经节 细胞培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]