IMMOBILIZATION OF AMINOACYLASE ON MODIFIED METHYL ACRYLATE-DIVINYL BENZENE COPOLYMERS  

作　　者：LI Minqin HE Binglin(Institute of Polymer Chemistry, Naikai University, Tianjin 300071, China) 

出　　处：《Chinese Journal of Reactive Polymers》1993年第1期34-41,共8页中国反应性高分子（英文版）

摘　　要：Macroporous methyl acrylate-divinyl benzene copolymers were synthesized and modified by polyethylene polyamine.The aminoacylase from Aspergillus oryzae was adsorded on these modified copolymers.The role of certain factors such as degree of cross-linking,amount of poregenic solvent,modifying agent and pore structures,in the activity of immobilized aminoacylase was studied.Effects of adsorption time and amount of enzyme used on the activity of aminocylase immobilized on MMD-12-13 were also investigated.The column of aminoacylase immobilized on MMD-12-13 was prepared and N-acyl-DL-methionine was resolved continuously for a month,the remaining activity was still over 90%.Macroporous methyl acrylate - divinyl benzene copolymers were synthesized and modified by polyethylene polyamine. The aminoacylase from Aspergillus o-ryzae was adsorded on these modified copolymers. The role of certain factors such as degree of cross-linking, amount of poregenic solvent, modifying agent and pore structures, in the activity of immobilized aminoacylase was studied. Effects of adsorption time and amount of enzyme used on the activity of aminoacylase immobilized on MMD-12-13 were also investigated. The column of aminoacylase immobilized on MMD-12-13 was prepared and N-acyl-DL-methionine was resolved continuously for a month, the remaining activity was still over 90%.

关 键 词：氨基羰基反应定位 丙烯酸甲酯 二乙烯基聚芥聚物 

分 类 号：O632.13[理学—高分子化学] O632.14[理学—化学]