Xanthine Biosensor Based on Didodecyldimethylammonium Bromide Modified Pyrolytic Graphite Electrode  

作　　者：唐纪琳 韩晓军 黄卫民 汪尔康 

出　　处：《Chinese Journal of Chemistry》2002年第3期263-266,共4页中国化学（英文版）

基　　金：ProjectsupportedbytheNationalNaturalScienceFoundationofChina (No.2 9835 12 0 )

摘　　要：The vesicle of didodecyldimethylammonium bromide (DDAB) which containedtetrathiafulvalene (TTF) was mixed with xanthine oxidase, and the mixture was cast on the pyrolyticgraphite electrode. The lipid films were used to supply a biological environment resemblingbiomembrane on the surface of the electrode. TTF was used as a mediator because of its highelectron-transfer efficiency. A novel xanthine biosensor based on cast DDAB film was developed. Theeffects of pH and operating potential were explored for optimum analytical performance by using theamperometric method. The response time of the biosensor was less than 10 s. The detection limit ofthe biosensor was 3.2 x 10^(-7) mol/L and the liner range was from 4 x 10^(-7) mol/L to 2.4 x10^(-6) mol/L.The vesicle of didodecyldimethylammonium bromide (DDAB) which containedtetrathiafulvalene (TTF) was mixed with xanthine oxidase, and the mixture was cast on the pyrolyticgraphite electrode. The lipid films were used to supply a biological environment resemblingbiomembrane on the surface of the electrode. TTF was used as a mediator because of its highelectron-transfer efficiency. A novel xanthine biosensor based on cast DDAB film was developed. Theeffects of pH and operating potential were explored for optimum analytical performance by using theamperometric method. The response time of the biosensor was less than 10 s. The detection limit ofthe biosensor was 3.2 x 10^(-7) mol/L and the liner range was from 4 x 10^(-7) mol/L to 2.4 x10^(-6) mol/L.

关 键 词：didodecyldimethylammonium bromide (DDAB) BIOSENSOR xanthine oxidase (XOD) tetrathiafulvalene (TTF) 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]