Effects of Hydrophobicity of Ethylene Oxide-Propylene Oxide Copolymers on Phase Diagrams of Aqueous Two-Phase Systems and Partition Behaviors of Cephalexin and 7-Aminodeacetoxicephalos-poranic Acid  

作　　者：朱建航 魏东芝 曹学君 

机构地区：[1]StateKeyLaboratoryofBioreactorEngineering,InstituteofBiochemistry,EastChinaUniversityofScienceandTechnology,Shanghai200237,China

出　　处：《Chinese Journal of Chemical Engineering》2002年第2期240-243,共4页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No. 29873014) and the Key Disciplinary Foundation of Shanghai.

摘　　要：A series of ethylene oxide (EO)-propylene oxide (PO) randomco-polymers (EOPO) were used to form aqueous two-phase systems (ATPS)with ammonium sulfate. Effects of EOPO's properties on the phaseseparation behaviors and on the partition of cephalexin and7-aminodesacetoxicephalosporanic acid (7-ADCA) in ATPS wereinvestigated. Both the molar mass and molar ratio of EO to PO of EOPOcould greatly influence partition behaviors of cephalexin and 7-ADCAas well as the binodal curve of ATPS.A series of ethylene oxide(EO)-propylene oxide (PO) random co-polymers (EOPO) were used to form aqueous two-phase systems (ATPS) with ammonium sulfate.Effects of EOPO′s properties on the phase separation behaviors and on the partition of cephalexin and 7-aminodesacetoxicephalosporanic acid(7-ADCA) in ATPS were investigated.Both the molar mass and molar ratio of EO to PO of EOPO could greatly influence partition behaviors of cephalexin and 7-ADCA as well as the binodal curve of ATPS. With the increase of molar mass of co-polymer or the decrease of molar ratio of EO to PO,the critical point of ATPS decreased,the binodal curve became more asymmetry,and both cephalexin and 7-ADCA followed the same tendency to partition into the polymer-poor bottom phase.The experimental results shows that it is feasible to partition cephalexin and 7-ADCA in either the polymer-rich top phase or the polymer-poor bottom phase by choosing a specific phase-forming EOPO.

关 键 词：ethylene oxide (EO) and propylene oxide (PO) random co-polymer (EOPO) aqueous two-phase sys-tems partition coefficient phase diagram 

分 类 号：TQ316.3[化学工程—高聚物工业]