Spectrophotometric Study on the Interaction between Arsenazo M and Proteins  

作　　者：QiuLuanHU FengLinZHAO 等 

机构地区：[1]DpeartmentofChemistry，LuoYangteachersCollege,HeNan471022 [2]DepartmentofChemistry，PekingUniversity,Beijing100871

出　　处：《Chinese Chemical Letters》2002年第1期71-72,共2页中国化学快报（英文版）

摘　　要：Arsenazo M could bind with bovine serum albumin to form a complex in Clark-Lube buffer at pH 2.3 and room temperature, which gives a maximum absorption peak at 625 nm with a red shift of 75 nm compared with that of Arsenazo M itself. The apparent molar absorptivity of the BSA-Arsenazo M complex is 3.21105 Lmol-1cm-1. The linear ranges for protein determination are wide (at least 0-100 mg/mL).Arsenazo M could bind with bovine serum albumin to form a complex in Clark-Lube buffer at pH 2.3 and room temperature, which gives a maximum absorption peak at 625 nm with a red shift of 75 nm compared with that of Arsenazo M itself. The apparent molar absorptivity of the BSA-Arsenazo M complex is 3.21105 Lmol-1cm-1. The linear ranges for protein determination are wide (at least 0-100 mg/mL).

关 键 词：Arsenazo M serum proteins spectrophotometry. 

分 类 号：O629.73[理学—有机化学] O657[理学—化学]