A Spectrometric Setup for Synchronous Total Internal Reflection Fluorescence Measurement at the Solid/Liquid Interface  

作　　者：Yao Qun LI Jia Ju XU Run Tang WANG Li Jun YU Zhao LI 

机构地区：[1]Department of Chemistry and the Key Laboratory of Analytical Sciences of MOE,Xiamen University,Xiamen 361005

出　　处：《Chinese Chemical Letters》2002年第6期571-572,共2页中国化学快报(英文版)

摘　　要：A spectrometric setup to perform total internal reflection fluorescence (TIRF) and synchronous TIRF measurements at solid/liquid interfaces is presented. The combination of TIRF and synchronous fluorescence was proposed to analyze simultaneously different components at interfaces. The TIRF excitation, emission and synchronous spectra of a watersoluble porphyrin were obtained from water/glass interface using this setup without the existence of a surfactant.A spectrometric setup to perform total internal reflection fluorescence (TIRF) and synchronous TIRF measurements at solid/liquid interfaces is presented. The combination of TIRF and synchronous fluorescence was proposed to analyze simultaneously different components at interfaces. The TIRF excitation, emission and synchronous spectra of a watersoluble porphyrin were obtained from water/glass interface using this setup without the existence of a surfactant.

关 键 词：Total internal reflection fluorescence synchronous fluorescence spectrofluorimeter meso-tetra(4-sulfonatophenyl)porphyrin. 

分 类 号：O657.3[理学—分析化学]