一种乙肝病毒表面抗原和其单克隆抗体相互作用的SERS研究  

Surface-Enhanced Raman Spectroscopic Study on the Interaction of Hepatitis B Virus Surface Antigen and Its Monoclonal Antibody

作　　者：王连英[1] 纪小会[1] 袁航[2] 马岚[2] 孔祥贵[3] 刘益春[3] 杨文胜[1] 白玉白[1] 李铁津 

机构地区：[1]吉林大学化学学院,长春130023 [2]云南大学单克隆抗体工程技术研究中心,昆明650091 [3]中国科学院长春光学精密机械与物理所,长春130021

出　　处：《化学学报》2002年第12期2115-2119,共5页Acta Chimica Sinica

摘　　要：利用银纳米粒子的表面增强拉曼散射(SERS)效应,研究了乙肝病毒表面抗原(HBsAg)和其鼠源单克隆抗体(单抗,Ab HBsAg)的相互作用.SERS光谱结果表明,Ab HBsAg分子主要通过位于非抗原结合部位的去质子化羧基(COO-)实现与银纳米粒子的结合.HBsAg与Ab HBsAg相互作用形成免疫复合物后,HBsAg分子上的色氨酸(Trp)残基特征振动完全消失,表明Trp残基位于HBsAg抗原分子的活性区,是HBsAg与AbSurface-enhanced Raman scattering (SERS) has been employed to study the interaction of hepatitis B virus surface antigen (HBsAg) with mouse monoclonal antibody against HBsAg (Ab-HBsAg). SERS is used to obtain spectra of HBsAg, Ab-HBsAg and their immune complex adsorbed on colloidal silver. It is found that Ab-HBsAg is bound to silver colloids through the carboxyl groups, which are not on the antigen binding site of the Ab-HBsAg. The vibrations from tryptophan residues of the HBsAg disappear after formation of the immune complex. It is assumed that the tryptophan residues are among the antigen active sites of the HBsAg and play an important role in the interaction between Ab-HBsAg and HBsAg.

关 键 词：乙肝病毒 表面抗原 单克隆抗体 相互作用 SERS 表面增强拉曼散射 银纳米粒子 

分 类 号：R346[医药卫生—基础医学]