细菌视紫红质/聚乙烯醇复合膜的制备及相关功能研究  

Preparation and Study of Bacteriorhodopsin/Poly(Vinyl Alcohol) Composite Films

作　　者：刘嘉[1] 明明[1] 刘坚[1] 黄力[1] 李庆国[2] 丁建东[1] 

机构地区：[1]复旦大学高分子科学系,聚合物分子工程教育部重点实验室,上海200433 [2]复旦大学生命科学院生理学与生物物理学系,上海200433

出　　处：《化学学报》2002年第12期2209-2213,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.2 982 5 10 9;2 0 1740 0 6);教育部高等学校优秀青年教师教学和科研奖励基金;国家 863项目和 973项目资助

摘　　要：细菌视紫红质(bR)是一种独特的光敏蛋白,具有光致变色和光驱质子泵功能.将bR蛋白包埋于聚乙烯醇(PVA)基质中,制备了bR/PVA复合膜.利用紫外 -可见分光光度计和自制的毫秒级动力学光谱仪,检测了样品的吸收光谱和光循环M中间体在脉冲光激发下随时间的变化;同时,利用凝胶扫描成像仪及相关分析软件考察了样品成膜后的均匀程度.实验表明:bR/PVA复合膜具有良好的均匀性、透明性和力学性能,而且bR蛋白保持了原有的生物活性和光学性质,bR与M中间体之间能达到一种光可控制的双稳态,M中间体的寿命也得到了显著的延长。Bacteriorhodopsin (bR) is a photosensitive protein, which acts as a photochromic material as well as a light-driven proton pump. A bR was embedded in polyvinyl alcohol to prepare for optical films. The absorption spectrum and the decay of M intermediate in the photocycle of bR were measured. The uniformity of the samples was detected. The bR/PVA films exhibit high uniformity, transparence and strength, while bR in films maintains its biologic activities and optic response. In addition, the measurements support a bistable-state model between the native state and M intermediate. In comparison with the case of bR in aqueous suspension, the decay of M intermediate is prolonged obviously for bR in PVA films, which might be beneficial for information-storage applications.

关 键 词：聚乙烯醇 制备 细菌视紫红质 光循环 光驱质子泵 聚合物复合膜 信息材料 信息存储 

分 类 号：O657.3[理学—分析化学]