苏云金杆菌4.0718菌株的发酵上清液中超氧化物歧化酶研究  

Purification and Properties of Superoxide Dismutase from the Supernatant of Bacillus thuringiensis 4.0718 Fermentation Culture

作　　者：李伟[1] 夏立秋[2] 王先酉[3] 邹国林[1] 

机构地区：[1]武汉大学生命科学学院,中国武汉430072 [2]湖南师范大学生命科学学院,中国长沙410006 [3]湖南师范大学医学院,中国长沙410006

出　　处：《湖南师范大学自然科学学报》2002年第4期59-63,共5页Journal of Natural Science of Hunan Normal University

摘　　要：将苏云金杆菌4.0718进行液体发酵培养,从发酵上清液中可以抽提得到SOD.经(NH4)2SO4分级沉淀、SephadexG 75凝胶过滤和HPLC3步纯化得到均一性SOD,其比活力达1040U mg.经检测该酶属Fe SOD,分子量为39486D,由2个相同亚基组成,其N 末端氨基酸为丙氨酸.讨论了发酵上清液中SOD与晶体蛋白杀虫毒力的关系.Superoxide dismutase (SOD) could be extracted from fermentative supermatant liquid of Bacillus thuringiensis 4.0718 fermentation culture. The enzyme was purified by a combination of ammonium sulfate fractionation, Sephadex G-75 gel filtration and reversed-phase high-performance liquid chromatography. The purified enzyme was a highly homogeneous protein with the specific activity of 1 040 U/mg. The enzyme has a molecular weight of 39 486 D with two equally sized subunits. The enzyme was an iron superoxide dismutase with N-terminal amino acid of alamine. The relation of SOD from fermentative supermatant liquid and the toxicity of parasporal crystals was discussed in this paper.

关 键 词：4.0718菌株 超氧化物歧化酶 苏云金杆菌 发酵上清液 液体发酵培养 微生物杀虫剂 

分 类 号：TQ453.5[化学工程—农药化工]