磷酸羟基哌喹对大鼠放射性肺纤维化的防治作用  

Radiation-induced fibrosis in rat lung: Modification by hydroxypiperquin phosphate

作　　者：王梅[1] 傅强[1] 闵碧荷[1] 肖作平[1] 

机构地区：[1]第二军医大学附属长海医院肿瘤科,上海200433

出　　处：《中国癌症杂志》2002年第6期519-521,共3页China Oncology

摘　　要：目的:研究磷酸羟基哌喹(hydroxypiperquin phosphate,HPQP)对大鼠放射性肺纤维化的防治作用。方法:选雄性成年SD大鼠给予0、15 Gy、20 Gy、25 Gy四种剂量60Co γ射线单次半胸照射。各剂量组再分服药(HPQP)组与不服药组(对照组)。照射后4个月和6个月两个时间点对各组的肺组织羟脯氨酸(HP)含量及肺病理形态变化(光镜和电镜)进行研究。结果:照射剂量愈大,肺组织HP含量亦愈高,其中20 Gy、25 Gy组与对照组相比有非常显著差别(P<0.01),而服药组肺HP含量明显低于不服药组(P<0.01)。病理形态变化(肺泡壁增厚、胶原增多、肺泡腔内巨噬细胞和泡沫样细胞出现、肺毛细血管腔狭窄、闭塞等)亦随着照射剂量的加大而愈趋严重,服药组可明显减轻上述改变。结论:证实HPQP可抑制胶原形成和减轻照射所引起的病理变化,对大鼠放射性肺纤维化具有一定的防治作用,并复制了放射性肺纤维化的动物模型。Purpose: To evaluate the HPQP as a modifier of radiation-induced pulmonary fibrosis in rats sacrificed 4 months and 6 months separately after a single dose of 0, 15, 20, 25 Gy60Co r rays to the right hemithorax. Methods: HPQP was administered by a stomach tube 2 times a week after irradiation at a regimen of 100mg/kg. Pulmonary fibrosis was evaluated by lung hydroxyproline( HP) content and histological observation( LM and EM). Results: The content of lung HP increased with increasing radiation dose. The differences between control group and 20,25 Gy group are significant P <0. 01). Cotreatment with HPQP significantly ameliorated this response (P < 0. 01). The intensity of pulmonary pathological changes increased with increasing radiation dose also, and HPQP can lessen these changes greatly. Conclusions: HPQP may inhibit the progression of lung fibrosis and that the clinical application of HPQP is somehow evidenced by this study. This experiment also established an animal model of radiation-induced pulmonary fibrosis successfully.

关 键 词：肺纤维化 磷酸羟基哌喹 放射性肺纤维化 大鼠 羟脯氨酸 病理变化 

分 类 号：R563.105[医药卫生—呼吸系统]